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Our Abpromise guarantee covers the use of ab34616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Immunomicroscopy||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. PubMed: 45957|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.|
|ELISA||Use at an assay dependent concentration.|
ab34616 staining Asparaginase in Mice treated with E.coli Asparaginase or mock treated mice were sacrificed 16 hours after injection. Tissue was stained for Asparaginase uptake by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 2% Normal Goat serum + 2% BSA for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1500 in blocking buffer) for 1 hours at 20°C.
ab34616 has not yet been referenced specifically in any publications.