Anti-Asparaginase 抗体 (Biotin) (ab34616)


  • 製品名Anti-Asparaginase antibody (Biotin)
    Asparaginase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Asparaginase (Biotin)
  • 標識Biotin
  • アプリケーション適用あり: Immunomicroscopy, IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Escherichia coli
  • 免疫原

    Asparaginase (Escherichia coli )


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)This antibody was purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野


Our Abpromise guarantee covers the use of ab34616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Immunomicroscopy Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. PubMed: 45957
WB Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
ELISA Use at an assay dependent concentration.


  • 関連性Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme.
  • 細胞内局在Cytoplasmic, Secreted and Periplasm.
  • 参照データベース
    • 別名
      • AnsA antibody
      • AnsB antibody
      • Colaspase antibody
      • Cytoplasmic asparaginase I antibody
      • L ASNase I antibody
      • L ASNase II antibody
      • L asparaginase 1 antibody
      • L asparaginase 2 antibody
      • L asparaginase I antibody
      • L asparaginase II antibody
      • L asparaginase II precursor antibody
      • L asparagine amidohydrolase I antibody
      • L asparagine amidohydrolase II antibody
      see all

    Anti-Asparaginase antibody (Biotin) 画像

    • ab34616 staining Asparaginase in Mice treated with E.coli Asparaginase or mock treated mice were sacrificed 16 hours after injection. Tissue was stained for Asparaginase uptake by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 2% Normal Goat serum + 2% BSA for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1500 in blocking buffer) for 1 hours at 20°C.

      See Abreview

    Anti-Asparaginase antibody (Biotin) (ab34616) 使用論文

    ab34616 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step (agent) for 30 minute(s) · Concentration: 2% · Temperature: 20°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate, pH6
    Sample Mouse Tissue sections (Liver)
    Specification Liver
    Permeabilization No
    Fixative Formaldehyde

    Abcam user community

    Verified customer

    投稿 Oct 31 2014

    Thank you for your enquiry. I have contacted the originator of this antibody and unfortunately we do not know which asparaginase this antibody is specific to; we only know that it reacts against asparaginase in E. coli. I am sorry that we do not have m...

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