製品の概要

  • 製品名Anti-ASPA antibody
    ASPA 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ASPA
  • アプリケーション適用あり: ICC/IF, WB, IHC-P, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Cow, Pig
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 39-270 of Human ASPA (AAH29128).

  • ポジティブ・コントロール
    • 293T, A431, H1299, or HeLa cell lines. MOLT4 and Raji whole cell lysate. Cal27 xenograft IF/ICC: U-87 MG cell line.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 36 kDa.
IHC-P 1/100 - 1/500.
IP Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Catalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate. NAA occurs in high concentration in brain and its hydrolysis NAA plays a significant part in the maintenance of intact white matter. In other tissues it act as a scavenger of NAA from body fluids.
  • 組織特異性Brain white matter, skeletal muscle, kidney, adrenal glands, lung and liver.
  • 関連疾患Defects in ASPA are the cause of Canavan disease (CAND) [MIM:271900]; also known as spongy degeneration of the brain. CAND is a rare neurodegenerative condition of infancy or childhood characterized by white matter vacuolization and demeylination that gives rise to a spongy appearance. The clinical features are onset in early infancy, atonia of neck muscles, hypotonia, hyperextension of legs and flexion of arms, blindness, severe mental defect, megalocephaly, and death by 18 months on the average.
  • 配列類似性Belongs to the AspA/AstE family. Aspartoacylase subfamily.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACY 2 antibody
    • ACY-2 antibody
    • ACY2 antibody
    • ACY2_HUMAN antibody
    • Aminoacylase 2 antibody
    • Aminoacylase-2 antibody
    • Aminoacylase2 antibody
    • ASP antibody
    • ASPA antibody
    • Aspartoacylase (aminoacylase 2, Canavan disease) antibody
    • Aspartoacylase (Canavan disease) antibody
    • Aspartoacylase antibody
    • NUR 7 antibody
    • NUR7 antibody
    • OTTMUSP00000006437 antibody
    • RP23-213I10.1 antibody
    • Small lethargic antibody
    see all

Anti-ASPA antibody 画像

  • ab97454 stained U-87 MG cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97454 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • ASPA was immunoprecipitated using 0.5mg MOLT4 whole cell extract, 5µg of Rabbit polyclonal to ASPA and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, MOLT4 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab97454.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 36kDa, non specific bands - 42 and 51kDa: We are unsure as to the identity of this extra band; ASPA

  • All lanes : Anti-ASPA antibody (ab97454) at 1/1000 dilution

    Lane 1 : MOLT4 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 36 kDa
    10% SDS PAGE
  • ab97454 at 1/100 dilution staining ASPA in paraffin-embedded Cal27 xenograft by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-ASPA antibody (ab97454) 使用論文

ab97454 has not yet been referenced specifically in any publications.

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