Anti-Asialoglycoprotein Receptor 1 抗体 (ab88042)

製品の概要

  • 製品名
    Anti-Asialoglycoprotein Receptor 1 antibody
    Asialoglycoprotein Receptor 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Asialoglycoprotein Receptor 1
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Asialoglycoprotein Receptor 1.

    (Peptide available as ab98248.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HepG2 whole cell lysate and FFPE human liver tissue sections.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab88042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 33 kDa).

ターゲット情報

  • 機能
    Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.
  • 組織特異性
    Expressed exclusively in hepatic parenchymal cells.
  • 配列類似性
    Contains 1 C-type lectin domain.
  • 翻訳後修飾
    Phosphorylated on a cytoplasmic Ser residue.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ASGP-R 1 antibody
    • ASGPR 1 antibody
    • ASGPR antibody
    • ASGPR1 antibody
    • Asgr1 antibody
    • ASGR1_HUMAN antibody
    • Asialoglycoprotein receptor 1 antibody
    • C type lectin domain family 4 member H1 antibody
    • C-type lectin domain family 4 member H1 antibody
    • CLEC4H1 antibody
    • Hepatic lectin H1 antibody
    • HL-1 antibody
    see all

画像

  • Anti-Asialoglycoprotein Receptor 1 antibody (ab88042) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes
  • IHC image of ab88042 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88042, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献

This product has been referenced in:
  • Zhang D  et al. Simplified quantification method for in vivo SPECT/CT imaging of asialoglycoprotein receptor with (99m)Tc-p(VLA-co-VNI) to assess and stage hepatic fibrosis in mice. Sci Rep 6:25377 (2016). Read more (PubMed: 27150943) »

See 1 Publication for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepG2 cells)
Permeabilization
Yes - 0.2% Triton X-100
Specification
HepG2 cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 27 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

投稿 Oct 13 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100
Username

Dr. Kirk Mcmanus

Verified customer

投稿 Jun 13 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録