製品の概要

  • 製品名Anti-ASH2L antibody [2046D2a]
    ASH2L 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2046D2a] to ASH2L
  • アプリケーション適用あり: Flow Cyt, WB, ICC, Dot blotmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment

  • ポジティブ・コントロール
    • WB: HeLa, NIH3T3 or F2408 whole cell lysate. ICC/IF: HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/50. Detects a band of approximately 75-76 kDa (predicted molecular weight: 69 kDa).
ICC 1/5.
Dot blot Use at an assay dependent concentration.

ターゲット情報

  • 機能Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis.
  • 組織特異性Ubiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes.
  • 配列類似性Contains 1 B30.2/SPRY domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ASH 2 antibody
    • Ash2 (absent small or homeotic) like antibody
    • Ash2 (absent, small, or homeotic) like (Drosophila) antibody
    • ASH2 antibody
    • ASH2 LIKE antibody
    • ASH2 like protein antibody
    • ASH2-like protein antibody
    • Ash2l antibody
    • ASH2L_HUMAN antibody
    • ASH2L1 antibody
    • ASH2L2 antibody
    • Bre 2 antibody
    • Bre2 antibody
    • Discs 2-like antibody
    • Drosophila absent, small, or homeotic antibody
    • Drosophila ASH2-like antibody
    • Set1/Ash2 histone methyltransferase complex subunit ASH2 antibody
    see all

Anti-ASH2L antibody [2046D2a] 画像

  • Anti-ASH2L antibody [2046D2a] (ab50699) at 1/50 dilution + HeLa whole cell lysate at 25 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 69 kDa
    Observed band size : 76 kDa (why is the actual band size different from the predicted?)
  • HeLa cells labelled with ab50699 at 1/5 dilution.Secondary antibody: Alexa 488 Goat Anti-Mouse IgG at 1/200.
  • Anti-ASH2L antibody [2046D2a] (ab50699) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 69 kDa
    Observed band size : 76 kDa (why is the actual band size different from the predicted?)
  • Anti-ASH2L antibody [2046D2a] (ab50699) at 1/50 dilution + F2408 whole cell lysate at 50 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 69 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing HeLa cells stained with ab50699 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab50699, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-ASH2L antibody [2046D2a] (ab50699) 使用論文

This product has been referenced in:
  • McLaughlin N  et al. In situ histone landscape of nephrogenesis. Epigenetics 9:222-35 (2014). IHC-P ; Mouse . Read more (PubMed: 24169366) »
  • Benedikt A  et al. The leukemogenic AF4-MLL fusion protein causes P-TEFb kinase activation and altered epigenetic signatures. Leukemia 25:135-44 (2011). WB ; Human . Read more (PubMed: 21030982) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Mouse Cell (embryonic stem cells)
Specification embryonic stem cells
Permeabilization Yes - 0.5% TritonX-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 14 2014

Thank you for contacting Abcam.

The immunogen sequence of ab50699 as follows.



Unfortunately, we have not confirmed the exact epitope sequence that the antibody binds too.

Please let me know if there is anything ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 40 µg
Specification HEK293
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Dec 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"