製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ASF1A
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Chicken
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human ASF1A.

    (Peptide available as ab71590.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat, Staurosporine-Treated Jurkat (24hr, 500nM)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).

ターゲット情報

  • 機能
    Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Belongs to the ASF1 family.
  • 翻訳後修飾
    Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Anti silencing function 1A antibody
    • Anti-silencing function protein 1 homolog A antibody
    • ASF1 anti silencing function 1 homolog A (S. cerevisiae) antibody
    • ASF1 anti silencing function 1 homolog A antibody
    • asf1a antibody
    • ASF1A_HUMAN antibody
    • CCG1 interacting factor A antibody
    • CCG1-interacting factor A antibody
    • CGI 98 antibody
    • CIA antibody
    • hAsf1 antibody
    • hAsf1a antibody
    • hCIA antibody
    • Histone chaperone ASF1A antibody
    • HSPC146 antibody
    see all

画像

  • All lanes : Anti-ASF1A antibody (ab66754) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 21 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 39 kDa, 73 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 minutes


    The 21 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to ASF1a.

参考文献

ab66754 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton X100 in PBS
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

投稿 Nov 15 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lymphoma)
Specification
lymphoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

投稿 Jan 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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