製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ASF1A
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chicken, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human ASF1 alpha/beta.

    (Peptide available as ab37490.)

  • ポジティブ・コントロール
    • ab24171 gives a positive result in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line), Jurkat (Human T cell lymphoblast-like cell line), A431 (Human epithelial carcinoma cell line) and MCF-7 (Human breast adenocarcinoma cell line).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab24171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB 1/250. Detects a band of approximately 23, 33 kDa (predicted molecular weight: 23 kDa).

ターゲット情報

  • 機能
    Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Belongs to the ASF1 family.
  • 翻訳後修飾
    Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Anti silencing function 1A antibody
    • Anti-silencing function protein 1 homolog A antibody
    • ASF1 anti silencing function 1 homolog A (S. cerevisiae) antibody
    • ASF1 anti silencing function 1 homolog A antibody
    • asf1a antibody
    • ASF1A_HUMAN antibody
    • CCG1 interacting factor A antibody
    • CCG1-interacting factor A antibody
    • CGI 98 antibody
    • CIA antibody
    • hAsf1 antibody
    • hAsf1a antibody
    • hCIA antibody
    • Histone chaperone ASF1A antibody
    • HSPC146 antibody
    see all

画像

  • All lanes : Anti-ASF1A antibody (ab24171) at 1/250 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)
    Lane 4 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 23,33 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa (possible non-specific secondary antibody binding).
  • ICC/IF image of ab24171 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab24171, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

参考文献

ab24171 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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