ab24171 gives a positive result in the following whole cell lysates:
HeLa (Human epithelial carcinoma cell line),
Jurkat (Human T cell lymphoblast-like cell line),
A431 (Human epithelial carcinoma cell line) and
MCF-7 (Human breast adenocarcinoma cell line).
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
1/250. Detects a band of approximately 23, 33 kDa (predicted molecular weight: 23 kDa).
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Belongs to the ASF1 family.
Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase.
ASF1 typically shows up as a band at approximately 33 kDa band on Western blots but has a predicted molecular weight of 23kDa. Occasionally doublet or triplet bands can be observed due to phosphorylation differences.
ICC/IF image of ab24171 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab24171, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).