製品の概要
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製品名Anti-Ascl2 antibody
Ascl2 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Ascl2
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由来種Rabbit
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アプリケーション適用あり: WBmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Ascl2.
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ポジティブ･コントロール
- This antibody gave a positive signal in Human Placenta tissue lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab116699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa). Abcam recommends using milk as the blocking agent (3%). |
ターゲット情報
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機能AS-C proteins are involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system.
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組織特異性Expressed specifically in the extravillous trophoblasts of the developing placenta.
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配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
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細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 430 Human
- Entrez Gene: 17173 Mouse
- Entrez Gene: 24209 Rat
- SwissProt: Q99929 Human
- SwissProt: O35885 Mouse
- SwissProt: P19360 Rat
- Unigene: 152475 Human
- Unigene: 196417 Mouse
see all -
別名
- Achaete scute complex like 2 antibody
- Achaete scute homolog 2 antibody
- achaete-scute complex-like 2 antibody
see all
画像
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Western blot - Anti-Ascl2 antibody (ab116699)Anti-Ascl2 antibody (ab116699) at 1 µg/ml (Milk blocking - 3%) + Human placenta tissue lysate - total protein (ab29745) at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 22 kDa (why is the actual band size different from the predicted?)
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab116699 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
プロトコール
参考文献
ab116699 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"