Rabbit polyclonal to Ascl2
Mouse, Rat, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Ascl2.
This antibody gave a positive signal in Human Placenta tissue lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa). Abcam recommends using milk as the blocking agent (3%).
AS-C proteins are involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system.
Expressed specifically in the extravillous trophoblasts of the developing placenta.
Contains 1 basic helix-loop-helix (bHLH) domain.
Information by UniProt
Achaete scute complex like 2 antibody
Achaete scute homolog 2 antibody
achaete-scute complex-like 2 antibody
Western blot - Anti-Ascl2 antibody (ab116699)
Anti-Ascl2 antibody (ab116699) at 1 µg/ml (Milk blocking - 3%) + Human placenta tissue lysate - total protein (
) at 20 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size :
Observed band size :
22 kDa (
why is the actual band size different from the predicted?
Additional bands at :
37 kDa. We are unsure as to the identity of these extra bands.
Exposure time :
has not yet been referenced specifically in any publications.
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