製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Ascl2
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Ascl2.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Placenta tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab116699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa). Abcam recommends using milk as the blocking agent (3%).

ターゲット情報

  • 機能
    AS-C proteins are involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system.
  • 組織特異性
    Expressed specifically in the extravillous trophoblasts of the developing placenta.
  • 配列類似性
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Achaete scute complex like 2 antibody
    • Achaete scute homolog 2 antibody
    • achaete-scute complex-like 2 antibody
    • Achaete-scute homolog 2 antibody
    • ASCL2 antibody
    • ASCL2_HUMAN antibody
    • ASH-2 antibody
    • Ash2 antibody
    • bHLHa45 antibody
    • Class A basic helix-loop-helix protein 45 antibody
    • HASH2 antibody
    • Mash2 antibody
    see all

画像

  • Anti-Ascl2 antibody (ab116699) at 1 µg/ml (Milk blocking - 3%) + Human placenta tissue lysate - total protein (ab29745) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 20 kDa
    Observed band size: 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes


    This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab116699 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

プロトコール

参考文献

ab116699 has not yet been referenced specifically in any publications.

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