The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
Use at 2-5 µg/mg of lysate.
Transcription coactivator of nuclear receptors which functions in conjunction with CBP-p300 and SRC-1 and may play an important role in establishing distinct coactivator complexes under different cellular conditions. Plays a pivotal role in the transactivation of NF-kappa-B, SRF and AP1. Acts as a mediator of transrepression between nuclear receptor and either AP1 or NF-kappa-B. Plays a role in androgen receptor transactivation and in testicular function.
Nucleus. Cytoplasm. Cytoplasmic under conditions of serum deprivation.
All lanes : Anti-ASC1 antibody (ab70627) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 66 kDa Observed band size: 66 kDa
Exposure time: 10 seconds
Immunoprecipitation - ASC1 antibody (ab70627)
Immunoprecipitation of HeLa whole cell lysate (1mg) using ab70627 at 3 µg/ml (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was used for Western blotting, analysed using ab70627 at 1µg/ml and detected by chemiluminescence for 10 seconds.