製品の概要

  • 製品名Anti-ASC-2 antibody
    ASC-2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ASC-2
  • 特異性This antibody was tested on U937, human prostate, human ovary and human testis extracts in western blot analysis and did not detect ASC-2 in these extracts.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human ASC-2.

  • ポジティブ・コントロール
    • This antibody gives a positive signal in HeLa, HepG2 and MCF7 nuclear lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab18193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 220 kDa).

ターゲット情報

  • 関連性The protein encoded by this gene is a nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone dependent fashion. It is involved in the coactivation of different nuclear receptors, such as steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). It probably functions as a general coactivator, rather than just a nuclear receptor coactivator,and may also be involved in the coactivation of the NFkB pathway (referenced from swissprot).
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • Activating signal cointegrator 2 antibody
    • AIB3 antibody
    • Amplified in breast cancer 3 protein antibody
    • Cancer amplified transcriptional coactivator ASC 2 antibody
    • NCOA6 antibody
    • Nuclear receptor coactivator 6 antibody
    • Nuclear receptor coactivator RAP250 antibody
    • PPAR interacting protein antibody
    • Thyroid hormone receptor binding protein antibody
    see all

Anti-ASC-2 antibody 画像

  • All lanes : Anti-ASC-2 antibody (ab18193) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 220 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 210 kDa,65 kDa,85 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab18193 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-ASC-2 antibody (ab18193) 使用論文

ab18193 has not yet been referenced specifically in any publications.

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