The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報WB: Use at a concentration of 1-5 µg/ml.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
機能Degrades bioactive fatty acid amides to their corresponding acids, with the following preference: N-palmitoylethanolamine > N-myristoylethanolamine > N-lauroylethanolamine = N-stearoylethanolamine > N-arachidonoylethanolamine > N-oleoylethanolamine. Also exhibits weak hydrolytic activity against the ceramides N-lauroylsphingosine and N-palmitoylsphingosine.
組織特異性Expressed in numerous tissues, with highest levels in liver and kidney, followed by pancreas.
配列類似性Belongs to the acid ceramidase family.
翻訳後修飾N-glycosylated. Tunicamycin treatment causes a reduction in specific activity against N-palmitoylethanolamine. Autoproteolytic cleavage in the acidic lumen of the lysosome activates the enzyme.