製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ASAH2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide surrounding amino acid 741 of human ASAH2

  • ポジティブ・コントロール
    • Jurkat cell lysate.

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 30% Glycerol, 0.5% BSA, PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab63804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).

ターゲット情報

  • 機能
    Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract.
  • 組織特異性
    Primarily expressed in the intestine (PubMed:17334805). Ubiquitously expressed with higher levels in kidney, skeletal muscle and heart (PubMed:10781606). According to PubMed:17334805, ubiquitous expression attributed to ASAH2 may be actually that of the paralog ASAH2B.
  • 配列類似性
    Belongs to the neutral ceramidase family.
  • 翻訳後修飾
    N-glycosylated. Required for enzyme activity.
    O-glycosylated. Required to retain it as a type II membrane protein at the cell surface.
    Phosphorylated. May prevent ubiquitination and subsequent degradation.
    Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by nitric oxid.
  • 細胞内局在
    Cell membrane. The neutral ceramidase soluble form is a secreted protein. According to PubMed:10781606, it is mitochondrial. However, they used a shorter form in its N-terminus, which may explain this localization which probably does not exist in vivo.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Acylsphingosine deacylase 2 antibody
    • AI585898 antibody
    • ASAH2 antibody
    • ASAH2_HUMAN antibody
    • BCDase antibody
    • hCD antibody
    • HNAC1 antibody
    • LCDase antibody
    • MGC129777 antibody
    • mitochondrial ceramidase antibody
    • N acylsphingosine amidohydrolase (non lysosomal ceramidase) 2 antibody
    • N acylsphingosine amidohydrolase 2 antibody
    • N CDase antibody
    • N-acylsphingosine amidohydrolase 2 antibody
    • N-CDase antibody
    • NCDase antibody
    • Neutral ceramidase soluble form antibody
    • Non lysosomal ceramidase antibody
    • Non-lysosomal ceramidase antibody
    see all

画像

  • All lanes : Anti-ASAH2 antibody (ab63804) at 4 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate


    Predicted band size : 86 kDa
    Observed band size : 86 kDa
    Additional bands at : 68 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab63804 has not yet been referenced specifically in any publications.

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