Anti-ASAH2 抗体 (ab113369)
Key features and details
- Rabbit polyclonal to ASAH2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ASAH2 antibody
ASAH2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ASAH2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide containing 17 residues from the C-terminal region of Human ASAH2.
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ポジティブ・コントロール
- Human small intestine tissue; 293 cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113369の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 86 kDa.
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IHC-P |
Use a concentration of 5 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 86 kDa. |
IHC-P
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. -
組織特異性
Primarily expressed in the intestine (PubMed:17334805). Ubiquitously expressed with higher levels in kidney, skeletal muscle and heart (PubMed:10781606). According to PubMed:17334805, ubiquitous expression attributed to ASAH2 may be actually that of the paralog ASAH2B. -
配列類似性
Belongs to the neutral ceramidase family. -
翻訳後修飾
N-glycosylated. Required for enzyme activity.
O-glycosylated. Required to retain it as a type II membrane protein at the cell surface.
Phosphorylated. May prevent ubiquitination and subsequent degradation.
Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by nitric oxid. -
細胞内局在
Cell membrane. The neutral ceramidase soluble form is a secreted protein. According to PubMed:10781606, it is mitochondrial. However, they used a shorter form in its N-terminus, which may explain this localization which probably does not exist in vivo. - Information by UniProt
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参照データベース
- Entrez Gene: 56624 Human
- Entrez Gene: 653308 Human
- Entrez Gene: 54447 Mouse
- Entrez Gene: 114104 Rat
- Omim: 611202 Human
- SwissProt: Q9NR71 Human
- SwissProt: Q9JHE3 Mouse
- SwissProt: Q91XT9 Rat
see all -
別名
- Acylsphingosine deacylase 2 antibody
- AI585898 antibody
- ASAH2 antibody
see all
画像
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab113369 は論文での使用が確認できていません。