製品の概要

  • 製品名Anti-ASAH1 antibody
    ASAH1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ASAH1
  • アプリケーション適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide of 16 amino acids taken from near the C-terminus of human ASAH1. (EAW63795).

  • ポジティブ・コントロール
    • Mouse heart tissue lysate
  • 特記事項

    Multiple isoforms of ASAH1 are known to exist.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab74469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 52 kDa.
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 2.5 µg/ml.

ターゲット情報

  • 機能Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.
  • 組織特異性Broadly expressed with highest expression in heart.
  • 関連疾患Defects in ASAH1 are the cause of Farber lipogranulomatosis (FL) [MIM:228000]; also known as Farber disease (FD). This sphingolipid disease is characterized by subcutaneous lipid-loaded nodules, excruciating pain in the joints and extremities, marked accumulation of ceramide in lysosomes, and death by three years of age.
  • 配列類似性Belongs to the acid ceramidase family.
  • 細胞内局在Lysosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AC antibody
    • ACDase antibody
    • Acid CDase antibody
    • Acid ceramidase antibody
    • Acid ceramidase precursor antibody
    • Acid ceramidase subunit beta antibody
    • Acylsphingosine deacylase antibody
    • ASAH 1 antibody
    • ASAH antibody
    • ASAH1 antibody
    • ASAH1_HUMAN antibody
    • FLJ21558 antibody
    • FLJ22079 antibody
    • N acylsphingosine amidohydrolase (acid ceramidase) 1 antibody
    • N acylsphingosine amidohydrolase 1 antibody
    • N acylsphingosine amidohydrolase antibody
    • N-acylsphingosine amidohydrolase antibody
    • N-acylsphingosine deacylase antibody
    • PHP antibody
    • PHP32 antibody
    • Putative 32 kDa heart protein antibody
    • SMAPME antibody
    see all

Anti-ASAH1 antibody 画像

  • Lane 1 : Anti-ASAH1 antibody (ab74469) at 1 µg/ml
    Lane 2 : Anti-ASAH1 antibody (ab74469) at 2 µg/ml

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Mouse heart tissue lysate

    Lysates/proteins at 15 µg per lane.


    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
  • ab74469 at 2.5µg/ml staining ASAH1 in human heart tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • Immunofluorescence of ASAH1 in Human Heart cells using ab74469 at 20 ug/ml.

Anti-ASAH1 antibody (ab74469) 使用論文

ab74469 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (Adrenal carcinoma)
Specification Adrenal carcinoma
Treatment Stable transfection with ASAH1-EGFP or EGFP
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jun 24 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"