製品の概要

  • 製品名Anti-Artemis antibody
    Artemis 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to Artemis
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human Artemis aa 316-577 (C terminal).
    Sequence:

    QTPGCCRAECMQSSRFTNFVDCEESNSESEEEVGIPASLQGDLGSVLHLQ KADGDVPQWE VFFKRNDEITDESLENFPSSTVAGGSQSPKLFSDSDGE STHISSQNSSQSTHITEQGSQG WDSQSDTVLLSSQERNSGDITSLDKA DYRPTIKENIPASLMEQNVICPKDTYSDLKSRDK DVTIVPSTGEPTTL SSETHIPEEKSLLNLSTNADSQSSSDFEVPSTPEAELPKREHLQYL YE KLATGESIAVKKRKCSLLDT

  • 特記事項During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Predicted molecular weight: 69 kDa.

ターゲット情報

  • 機能Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein exhibits single-strand specific 5'-3' exonuclease activity in isolation and acquires endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ.
  • 組織特異性Ubiquitously expressed, with highest levels in the kidney, lung, pancreas and placenta (at the mRNA level). Expression is not increased in thymus or bone marrow, sites of V(D)J recombination.
  • 関連疾患Defects in DCLRE1C are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive with sensitivity to ionizing radiation (RSSCID) [MIM:602450]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Individuals affected by RS-SCID show defects in the DNA repair machinery necessary for coding joint formation and the completion of V(D)J recombination. A subset of cells from such patients show increased radiosensitivity.
    Defects in DCLRE1C are the cause of severe combined immunodeficiency Athabaskan type (SCIDA) [MIM:602450]. SCIDA is a variety of RS-SCID caused by a founder mutation in Athabascan-speaking native Americans, being inherited as an autosomal recessive trait with an estimated gene frequency of 2.1% in the Navajo population. Affected individuals exhibit clinical symptoms and defects in DNA repair comparable to those seen in RS-SCID.
    Defects in DCLRE1C are a cause of Omenn syndrome (OS) [MIM:603554]. OS is characterized by severe combined immunodeficiency associated with erythrodermia, hepatosplenomegaly, lymphadenopathy and alopecia. Affected individuals have elevated T-lymphocyte counts with a restricted T-cell receptor (TCR) repertoire. They also generally lack B-lymphocytes, but have normal natural killer (NK) cell function (T+ B- NK+).
  • 配列類似性Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.
  • 翻訳後修飾Phosphorylation on undefined residues by PRKDC may stimulate endonucleolytic activity on 5' and 3' hairpins and overhangs. PRKDC must remain present, even after phosphorylation, for efficient hairpin opening. Also phosphorylated by ATM in response to ionizing radiation (IR) and by ATR in response to ultraviolet (UV) radiation.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A SCID antibody
    • A SCID protein antibody
    • Artemis protein antibody
    • ASCID antibody
    • DCLRE1C antibody
    • DCLRE1C DNA cross link repair 1C antibody
    • DCLRE1C protein antibody
    • DCLREC1C antibody
    • DCR1C_HUMAN antibody
    • DNA cross link repair 1C antibody
    • DNA cross link repair 1C protein antibody
    • DNA cross-link repair 1C protein antibody
    • FLJ11360 antibody
    • FLJ36438 antibody
    • hSNM1C antibody
    • OTTHUMP00000045150 antibody
    • Protein A-SCID antibody
    • Protein ARTEMIS antibody
    • PSO2 homolog antibody
    • RS SCID antibody
    • SCIDA antibody
    • Severe combined immunodeficiency type a antibody
    • SNM1 homolog C antibody
    • SNM1 like protein antibody
    • SNM1-like protein antibody
    • SNM1C antibody
    see all

Anti-Artemis antibody 画像



  • Predicted band size : 69 kDa


    Western Blot Test: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1/2000, Goat anti-IgY-HRP dilution: 1/1000. Colorimetric method for signal development. Arrow points to DCLRE1C fusion protein.

Anti-Artemis antibody (ab14289) 使用論文

This product has been referenced in:
  • Mohapatra S  et al. Restoration of G1 chemo/radioresistance and double-strand-break repair proficiency by wild-type but not endonuclease-deficient Artemis. Nucleic Acids Res : (2011). WB ; Human . Read more (PubMed: 21531702) »

See 1 Publication for this product

Product Wall

Thanks for your enquiry. I checked with the originator of this antibody and recombinant protein was used as the immunogen, not a synthetic peptide. I have updated the online datasheet to reflect this and apologize for the mistake. Please let me know if...

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