製品の概要

  • 製品名
    Anti-ART4 antibody
  • 製品の詳細
    Rabbit polyclonal to ART4
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Sheep, Rabbit, Horse, Cow, Pig, Chimpanzee, African green monkey
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 179-228 (TLCYEVHYRT KDVHFNAYTG ATIRFGQFLS TSLLKEEAQE FGNQTLFTIF) of Human ART4 (NP_066549).

  • ポジティブ・コントロール
    • Human Fetal heart lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/312500.

ターゲット情報

  • 関連性
    ART4 is a member of the ADP ribosyltransferase gene family. Antigens of the Dombrock blood group system are located on the gene product, which is glycosylphosphatidylinosotol anchored to the erythrocyte membrane. Allelic variants, some of which lead to adverse transfusion reactions, are known.
  • 細胞内局在
    Cell membrane; Lipidbanchor, GPI anchor.
  • 参照データベース
  • 別名
    • ADP ribosyltransferase 4 (DO blood group) antibody
    • ADP ribosyltransferase 4 (Dombrock blood group) antibody
    • ADP ribosyltransferase 4 antibody
    • ART 4 antibody
    • CD297 antibody
    • CD297 antigen antibody
    • DO antibody
    • DOK1 antibody
    • Dombrock blood group carrier molecule antibody
    • Ecto ADP ribosyltransferase 4 antibody
    • Mono ADP ribosyltransferase 4 antibody
    • Mono(ADP ribosyl)transferase 4 antibody
    • NAD(P)(+) arginine ADP ribosyltransferase 4 antibody
    see all

Anti-ART4 antibody 画像

  • Anti-ART4 antibody (ab83342) at 1/1 dilution + fetal heart lysate at 10 µg/ml

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa

プロトコール

Anti-ART4 antibody (ab83342) 使用論文

ab83342 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
Yes - 0.2% Triton X-100
Specification
Heart
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

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投稿 Oct 26 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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