製品の概要

  • 製品名Anti-ART1 antibody
    ART1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ART1
  • アプリケーション適用あり: ICC/IF, ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide selected from the central region of human ART1 conjugated to KLH.

  • ポジティブ・コントロール
    • HepG2 cell line lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab71295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).Can be blocked with ART1 peptide (ab209887).

ターゲット情報

  • 関連性ART1 belongs to the Arg-specific ADP-ribosyltransferase family. It catalyzes the transfer of ADP-ribose from NAD+ to arginine residues in proteins.
  • 細胞内局在Sarcoplasmic reticulum membrane; Lipid-anchor, GPI-anchor.
  • 参照データベース
  • 別名
    • ADP ribosyltransferase 1 antibody
    • ADP ribosyltransferase 2 antibody
    • ART 1 antibody
    • ART2 antibody
    • CD296 antibody
    • CD296 antigen antibody
    • GPI linked NAD(P)(+) arginine ADP ribosyltransferase 1 antibody
    • MGC133217 antibody
    • Mono(ADP ribosyl)transferase antibody
    • RT6 antibody
    see all

Anti-ART1 antibody 画像

  • Anti-ART1 antibody (ab71295) at 1/13 dilution + HepG2 cell line lysate at 35 µg

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 24 kDa,30 kDa,40 kDa,60 kDa. We are unsure as to the identity of these extra bands.

Anti-ART1 antibody (ab71295) 使用論文

This product has been referenced in:
  • Fabrizio G  et al. ARTC1-mediated ADP-ribosylation of GRP78/BiP: a new player in endoplasmic-reticulum stress responses. Cell Mol Life Sci 72:1209-25 (2015). WB ; Human . Read more (PubMed: 25292337) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - saponin
Fixative Methanol
Username

Miss. Gaia Fabrizio

Verified customer

投稿 Oct 24 2014

Thank you for contacting us.

Here is the link to the database entry I was refering to
http://www.uniprot.org/uniprot/P52961

There you will find some information about the disulfide bonds as well as the glycosylation sites. Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"