Anti-ARSB 抗体 (ab85727)
Key features and details
- Goat polyclonal to ARSB
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ARSB antibody
ARSB 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to ARSB -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow, Cat -
免疫原
Synthetic peptide corresponding to Human ARSB (internal sequence) (Cysteine residue).
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ポジティブ・コントロール
- Skeletal muscle tissue; human heart lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85727の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 60 kDa.
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IHC-P |
Use a concentration of 5 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 60 kDa. |
IHC-P
Use a concentration of 5 µg/ml. |
ターゲット情報
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関連疾患
Defects in ARSB are the cause of mucopolysaccharidosis type 6 (MPS6) [MIM:253200]; also known as Maroteaux-Lamy syndrome. MPS6 is an autosomal recessive lysosomal storage disease characterized by intracellular accumulation of dermatan sulfate. Clinical features can include abnormal growth, short stature, stiff joints, skeletal malformations, corneal clouding, hepatosplenomegaly, and cardiac abnormalities. A wide variation in clinical severity is observed.
Arylsulfatase B activity is defective in multiple sulfatase deficiency (MSD) [MIM:272200]. MSD is a disorder characterized by decreased activity of all known sulfatases. MSD is due to defects in SUMF1 resulting in the lack of post-translational modification of a highly conserved cysteine into 3-oxoalanine. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. -
配列類似性
Belongs to the sulfatase family. -
翻訳後修飾
The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. This post-translational modification is severely defective in multiple sulfatase deficiency (MSD). -
細胞内局在
Lysosome. - Information by UniProt
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参照データベース
- Entrez Gene: 100216331 Cat
- Entrez Gene: 411 Human
- Omim: 611542 Human
- SwissProt: P33727 Cat
- SwissProt: P15848 Human
- Unigene: 149103 Human
- Unigene: 604199 Human
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別名
- Arsb antibody
- ARSB_HUMAN antibody
- Arylsulfatase B antibody
see all
画像
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ab85727, at 5 µg/ml, staining ARSB in skeletal muscle tissue by Immunohistochemistry.
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Anti-ARSB antibody (ab85727) at 0.03 µg/ml + human heart lysate at 35 µg
Predicted band size: 60 kDa
Observed band size: 35,40 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab85727 は 1 報の論文で使用されています。
- Luo M et al. Changes in the metabolism of chondroitin sulfate glycosaminoglycans in articular cartilage from patients with Kashin-Beck disease. Osteoarthritis Cartilage 22:986-95 (2014). PubMed: 24857976