製品の概要

  • 製品名Anti-Arrow antibody
  • 製品の詳細
    Mouse polyclonal to Arrow
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Drosophila melanogaster
  • 免疫原

    Vector coding for a partial recombinant fusion protein, corresponding to internal sequence amino acids 261-360 of Fruit fly (Drosophila melanogaster) Arrow Target sequence used to make antibody: PNGLAVDLKN ELIYWTDGKH HFIDVMRLDG SSRRTIVNNL KYPFSLTFYD DRLYWTDWQR GSLNALDLQT RELKELIDTP KAPNSVRAWD PSLQPYEDNP.

  • 特記事項

     

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab43432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 関連性Arrow is part of the Wnt signalling system, and plays a role in eye morphogenesis for Drosophila.
  • 細胞内局在Plasma membrane
  • 参照データベース
    • 別名
      • CG5912 antibody
      • LRP antibody
      • LRP/Arr antibody
      • LRP5/6 antibody
      • LRP6 antibody
      see all

    Anti-Arrow antibody (ab43432) 使用論文

    ab43432 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab43432.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"