製品の概要

  • 製品名Anti-Arp5 antibody
  • 製品の詳細
    Rabbit polyclonal to Arp5
  • アプリケーション適用あり: WB, ChIPmore details
  • 種交差性
    交差種: Saccharomyces cerevisiae
  • 免疫原

    Recombinant full length protein Arp 5.

  • ポジティブ・コントロール
    • S. cereviseae lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab12099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
ChIP Use at an assay dependent concentration.

ターゲット情報

  • 関連性The budding yeast INO80 complex is a conserved ATP-dependent nucleosome remodeler containing actin-related proteins Arp5 and Arp8. Strains lacking INO80, ARP5, or ARP8 have defects in transcription. Ino80, Arp5, and Arp8 are recruited to an HO-induced DSB, where a phosphorylated form of H2A accumulates. Recruitment of Ino80 is compromised in cells lacking the H2A phosphoacceptor S129. INO80-mediated chromatin remodeling seems to occur directly at DSBs, where it appears to facilitate processing of the lesion.
  • 細胞内局在Nuclear
  • 参照データベース
    • 別名
      • Actin-like protein ARP5 antibody
      • ARP5_YEAST antibody
      • P53946 antibody

    Anti-Arp5 antibody 画像

    • Anti-Arp5 antibody (ab12099) + S.cerevisiae lysate

      Secondary
      Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution

      Predicted band size : 87 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 70 kDa (possible cross reactivity).
    • All lanes : Anti-Arp5 antibody (ab12099) at 0.36 µg/ml

      Lane 1 : S. cerevisiae whole cell lysate (wild type)
      Lane 2 : S. cerevisiae whole cell lysate (arp5 knockout)
      Lane 3 : S. cerevisiae whole cell lysate (arp8 knockout)
      Lane 4 : S. cerevisiae whole cell lysate (ino80 knockout)
      Lane 5 : S. cerevisiae whole cell lysate (anc1 knockout)
      Lane 6 : S. cerevisiae whole cell lysate (nhp10 knockout)
      Lane 7 : S. cerevisiae whole cell lysate (wild type, treated with MMS)
      Lane 8 : S. cerevisiae whole cell lysate (arp8 knockout, treated with MMS)


      Predicted band size : 87 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)

    Anti-Arp5 antibody (ab12099) 使用論文

    This product has been referenced in:
    • Panday A  et al. Yeast high mobility group protein HMO1 stabilizes chromatin and is evicted during repair of DNA double strand breaks. Nucleic Acids Res 43:5759-70 (2015). ChIP . Read more (PubMed: 25979266) »
    • Bennett G  et al. DNA repair choice defines a common pathway for recruitment of chromatin regulators. Nat Commun 4:2084 (2013). Read more (PubMed: 23811932) »

    See all 2 Publications for this product

    Product Wall

    Application ChIP
    Detection step Other
    Sample Saccharomyces cerevisiae Cell lysate - whole cell (Whole cell lysate)
    Specification Whole cell lysate
    Negative control No Antibody sample
    Type Cross-linking (X-ChIP)
    Duration of cross-linking step: 20 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 37% Formaldehyde
    Positive control Sample after 1 hour of DNA double strand break
    Username

    Mr. Arvind Panday

    Verified customer

    投稿 Jul 09 2014

    Application Western blot
    Loading amount 3.75e+006 cells
    Gel Running Conditions Reduced Denaturing (NUPAGE precasted Invitrogen 10% SDS-PAGE)
    Sample Schizosaccharomyces pombe Cell lysate - whole cell (whole cell extract)
    Specification whole cell extract
    Blocking step 0.5% BSA + 5% non fat milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    投稿 Jul 17 2013

    Thank you for contacting us. I have received the following information from the lab: Ab12099 (ARP5): Unfortunately, the full WB image showing the knock-out experiment is not on file, thus we are not able to determine if there were a...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"