Rabbit polyclonal to Arp2 (phospho T237 + T238)
Mouse, Chicken, Fish
Synthetic phospho-peptide (T237 + T238), conjugated to KLH corresponding to amino acid residues surrounding threonine 237 and threonine 238 in Human Arp2.
Rat PC12 cells differentiated with NGF. Human A431 and Jurkat cells treated with Calyculin A.
Do not aliquot.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 32, 44 kDa (predicted molecular weight: 45 kDa).
Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament.
Belongs to the actin family. ARP2 subfamily.
Cytoplasm > cytoskeleton. Cell projection.
Information by UniProt
Actin like protein 2 antibody
Actin related protein 2 antibody
Actin-like protein 2 antibody
Western blot - Anti-Arp2 (phospho T237 + T238) antibody (ab119766)
All lanes : Anti-Arp2 (phospho T237 + T238) antibody (ab119766) at 1/1000 dilution Lane 1 : A431 cells treated with Calyculin A
(100 nM) for 30 min. Lane 2 : A431 cells treated with Calyculin A
(100 nM) for 30 min and treated with lambda phosphatase Predicted band size : 45 kDa
Immunocytochemistry - Anti-Arp2 (phospho T237 + T238) antibody (ab119766)
ab119766 at 1/100 dilution staining Arp2 (phospho T237 + T238) in rat PC12 cells differentiated with NGF (right).
Detection used an appropriate secondary antibody conjugated to Cy3.
has not yet been referenced specifically in any publications.
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