製品の概要

  • 製品名Anti-ARP10 antibody
    ARP10 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to ARP10
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-182 of Human ARP10

  • ポジティブ・コントロール
    • Human colon tissue lysate and transfected 293 cell line lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab73055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: 1/500 - 1/1000. Expected band of 21.5 kDa (Isoform 2). Detects a band of approximately 19/20 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性ARP10 belongs to the cytidine and deoxycytidylate deaminase family. It is a poorly expressed deaminase that is ineffective at inhibiting retroviral replication. ARP10 has DNA deaminase (cytidine deaminase) activity but is not able to mediate G-to-A hypermutation in newly synthesized retroviral DNA. It is present at low level in 293T cells. It is considered an unstable protein, suggesting that it may be degraded by the proteasome (at protein level). Weakly expressed in testis, ovary, fetal liver and skin. There are two named isoforms. ARP10 from old world monkeys has retained its antiviral activity, while it is lost in other primates. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22.
    • 細胞内局在Nucleus. Cytoplasm. Note: Mostly nuclear.
    • 参照データベース
    • 別名
      • A3H antibody
      • APOBEC related protein 10 antibody
      • APOBEC3H antibody
      • Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 3H antibody
      • ARP 10 antibody
      • DNA dC->dU-editing enzyme APOBEC-3H antibody
      see all

    Anti-ARP10 antibody 画像

    • Anti-ARP10 antibody (ab73055) at 1/500 dilution + human colon tissue lysate at 25 µg

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-ARP10 antibody (ab73055) at 1/500 dilution

      Lane 1 : ARP10 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Observed band size : 19 kDa (why is the actual band size different from the predicted?)

    Anti-ARP10 antibody (ab73055) 使用論文

    This product has been referenced in:

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"