Anti-A3H 抗体 (ab73055)
Key features and details
- Mouse polyclonal to A3H
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-A3H antibody -
製品の詳細
Mouse polyclonal to A3H -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human A3H aa 1-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
-
ポジティブ・コントロール
- Human colon tissue lysate and transfected 293 cell line lysate
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Constituents: Whole serum, 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab73055の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 19,20 kDa.
Expected band of 21.5 kDa (Isoform 2). |
特記事項 |
---|
WB
1/500 - 1/1000. Detects a band of approximately 19,20 kDa. Expected band of 21.5 kDa (Isoform 2). |
ターゲット情報
-
関連性
ARP10 belongs to the cytidine and deoxycytidylate deaminase family. It is a poorly expressed deaminase that is ineffective at inhibiting retroviral replication. ARP10 has DNA deaminase (cytidine deaminase) activity but is not able to mediate G-to-A hypermutation in newly synthesized retroviral DNA. It is present at low level in 293T cells. It is considered an unstable protein, suggesting that it may be degraded by the proteasome (at protein level). Weakly expressed in testis, ovary, fetal liver and skin. There are two named isoforms. ARP10 from old world monkeys has retained its antiviral activity, while it is lost in other primates. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. -
細胞内局在
Nucleus. Cytoplasm. Note: Mostly nuclear. -
参照データベース
- Entrez Gene: 164668 Human
- Omim: 610976 Human
- SwissProt: Q6NTF7 Human
-
別名
- A3H antibody
- APOBEC related protein 10 antibody
- APOBEC3H antibody
see all
画像
-
Anti-A3H antibody (ab73055) at 1/500 dilution + human colon tissue lysate at 25 µg
Secondary
Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Observed band size: 20 kDa why is the actual band size different from the predicted? -
All lanes : Anti-A3H antibody (ab73055) at 1/500 dilution
Lane 1 : A3H transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Observed band size: 19 kDa why is the actual band size different from the predicted?
データシートおよび資料
-
Datasheet download
参考文献 (1)
ab73055 は 1 報の論文で使用されています。
- Desimmie BA et al. APOBEC3 proteins can copackage and comutate HIV-1 genomes. Nucleic Acids Res 44:7848-65 (2016). PubMed: 27439715