製品の概要

法規制情報

製品の詳細

  • 由来Synthetic

関連製品

特性

Our Abpromise guarantee covers the use of ab51924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Blocking - Blocking peptide for Anti-Aromatase antibody (ab18995)

  • 精製度> 90 % SDS-PAGE.

  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: 0.02% Thimerosal (merthiolate)
    Constituents: 0.1% BSA, PBS, pH 7.2

関連情報

  • 別名
    • ARO
    • ARO1
    • Aromatase
    • CP19A_HUMAN
    • CPV1
    • CYAR
    • CYP19
    • Cyp19a1
    • CYPXIX
    • Cytochrome P-450AROM
    • Cytochrome P450 19A1
    • Cytochrome P450, family 19, subfamily A, polypeptide 1
    • Cytochrome P450, subfamily XIX (aromatization of androgens)
    • Estrogen synthase
    • Estrogen synthetase
    • Flavoprotein linked monooxygenase
    • MGC104309
    • Microsomal monooxygenase
    • OTTHUMP00000162543
    • OTTHUMP00000198350
    • P 450AROM
    see all
  • 機能Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
  • 組織特異性Brain, placenta and gonads.
  • 関連疾患Defects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
    Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries.
  • 配列類似性Belongs to the cytochrome P450 family.
  • 細胞内局在Membrane.
  • Information by UniProt

Aromatase peptide (ab51924) 使用論文

ab51924 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your message and for providing this further information.

I am sorry to hear suggestions and use of the blocking peptide have not improved the results on this occasion. It does appear as this vial of ab18995 is bad. I apprecia...

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Application Western blot

Review text: How long did you incubate the protein and antibody mix before adding it to the blot?
First Time: Incubation time of antibody and protein (peptide): two hour at room temperature
Second Time:Incubation time of antibody and protein (peptide): One hour at 37 degree C
How much antibody/protein mix was added to the blot?
Antibody used for the reaction is 1ug and protein (peptide) 5ug (1:5 ratio)
Sample: Mouse Tissue lysate - other (BRAIN)
Loading amount: 15 µg
Specification: BRAIN
Blocking step: Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details
Concentration: 5 µg/ml
Incubation time: 1 hour(s) and 0 minute(s) · Temperature: 30°C

Primary antibody (in addition to 'Aromatase peptide')
Concentration: 1 µg/ml

Secondary antibody
Name: Non-Abcam antibody was used: anti - rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/20000

Detection
Detection method: ECL
Exposure: 2 minute(s) and 0 second(s)
Bands: Specific: 54 kDa Non-specific: 104, 77, 71, 67, 46, 36, kDa

Additional data
Additional Notes: AROMATASE PEPTIDE DON'T BLOCK ANY BANDS.
Peptide usage 5 times - 1ug of antibody and 5ug of blocking peptide.
Abcam response: The blocking peptide was the same as the immunogen. At the moment we have not found a reason why blocking is not taking place,
We have not had similar complaints for Aromatase peptide (ab51924).
More blocking peptide might be needed in customer's case.
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Verified customer

投稿 Apr 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"