製品の概要

  • 製品名Anti-ARMET antibody
    ARMET 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ARMET
  • 特異性ab67271 does not crossreact with CDNF.
  • アプリケーション適用あり: IHC-Fr, ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A 12 amino acid synthetic peptide from near the carboxy terminus of human ARMET.

  • ポジティブ・コントロール
    • Mouse brain tissue lysate. Human brain tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab67271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. PubMed: 18078525
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 20 kDa).

ターゲット情報

  • 機能Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death.
  • 配列類似性Belongs to the ARMET family.
  • ドメインThe N-terminal region may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response.
  • 翻訳後修飾May contain sialic acid residues.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Arginine rich mutated in early stage tumors antibody
    • Arginine rich protein antibody
    • Arginine-rich protein antibody
    • ARMET antibody
    • ARMET protein antibody
    • ARP antibody
    • Manf antibody
    • MANF_HUMAN antibody
    • Mesencephalic astrocyte derived neurotrophic factor antibody
    • Mesencephalic astrocyte-derived neurotrophic factor antibody
    • MGC142148 antibody
    • MGC142150 antibody
    • Protein ARMET antibody
    see all

Anti-ARMET antibody 画像

  • Lane 1 : Anti-ARMET antibody (ab67271) at 1 µg/ml
    Lane 2 : Anti-ARMET antibody (ab67271) at 2 µg/ml

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    Anti rabbit secondary antibody

    Predicted band size : 20 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  • ab67271 at 2.5 μg/ml staining of ARMET in human brain tissue using IHC-P.
  • ICC/IF image of ab67271 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67271, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of MANF in Human Brain cells using ab67271 at 20 ug/ml.

Anti-ARMET antibody (ab67271) 使用論文

This product has been referenced in:
  • Vanhoutte D  et al. Thrombospondin expression in myofibers stabilizes muscle membranes. Elife 5:N/A (2016). WB ; Mouse . Read more (PubMed: 27669143) »
  • Krawczyk KK  et al. Assessing the contribution of thrombospondin-4 induction and ATF6a activation to endoplasmic reticulum expansion and phenotypic modulation in bladder outlet obstruction. Sci Rep 6:32449 (2016). WB ; Mouse . Read more (PubMed: 27581066) »

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"