Immunofluorescent staining of Human cell line U-251MG shows positivity in nuclear membrane, cytoplasm and centrosome. Recommended concentration of ab121448 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121448, at 1/225 dilution, staining ARL6IP6 in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
Western blot - Anti-ARL6IP6 antibody (ab121448)
All lanes : Anti-ARL6IP6 antibody (ab121448) at 1/250 dilution
Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2 : Negative control (vector only transfected HEK293T lysate) Lane 3 : ARL6IP6 transfected HEK293T over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))