The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
Use at an assay dependent concentration.
Involved in tolerance to glucose starvation. Phosphorylates ATM. Suppresses Fas-induced apoptosis by phosphorylation of CASP6, thus suppressing the activation of the caspase and the subsequent cleavage of CFLAR.
Expressed at high levels in heart and brain, and at lower levels in skeletal muscle, kidney, ovary, placenta, lung and liver.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 protein kinase domain.
Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.
ab37641, at 1/50 dilution, staining ARK5 in Human breast carcinoma by Immunohistochemistry, Formalin fixed, Paraffin embedded tissue. ab37641 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Peng JK et al. ?ypoxia-inducible factor 1-a promotes colon cell proliferation and migration by upregulating AMPK-related protein kinase 5 under hypoxic conditions. Oncol Lett15:3639-3645 (2018).
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