Anti-ARID2 抗体 [2036C1a] (ab51019)

製品の概要

  • 製品名
    Anti-ARID2 antibody [2036C1a]
    ARID2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2036C1a] to ARID2
  • アプリケーション
    適用あり: WB, ICC, Dot blotmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment

  • ポジティブ・コントロール
    • HeLa cells. HEK293 whole cell lysate. NIH3T3 whole cell lysate. F2408 whole cell lysate.
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab166850 as a replacement.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab51019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50. Detects a band of approximately 240 kDa (predicted molecular weight: 197 kDa).
ICC 1/5.
Dot blot Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). May be involved in targeting the complex to different genes. May be involved in regulating transcriptional activation of cardiac genes.
  • 組織特異性
    Highly expressed in heart and testis.
  • 配列類似性
    Contains 1 ARID domain.
    Contains 1 C2H2-type zinc finger.
    Contains 1 RFX-type winged-helix DNA-binding domain.
  • 発生段階
    Up-regulated in adult heart (at protein level).
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ARID domain containing protein 2 antibody
    • ARID domain-containing protein 2 antibody
    • ARID2 antibody
    • ARID2_HUMAN antibody
    • AT rich interactive domain 2 (ARID, RFX like) antibody
    • AT rich interactive domain containing protein 2 antibody
    • AT-rich interactive domain-containing protein 2 antibody
    • BAF antibody
    • BAF200 antibody
    • BRG1 associated factor 200 antibody
    • BRG1-associated factor 200 antibody
    • DKFZp686G052 antibody
    • DKFZp779P0222 antibody
    • FLJ30619 antibody
    • KIAA1557 antibody
    • p200 antibody
    • Zinc finger protein with activation potential antibody
    • zip zap antibody
    • zipzap antibody
    • Zipzap/p200 antibody
    see all

画像

  • Anti-ARID2 antibody [2036C1a] (ab51019) at 1/50 dilution + HEK293 whole cell lysate at 25 µg

    Secondary
    Mouse IgG anibody at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 197 kDa
    Observed band size : 240 kDa (why is the actual band size different from the predicted?)
  • HeLa cells stained with ab51019 at 1/5 dilution, followed by Alexa Fluor 488 Goat Anti-Mouse IgG at 1/200 dilution.
  • Anti-ARID2 antibody [2036C1a] (ab51019) at 1/50 dilution + NIH3T3 whole cell lysate at 25 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 197 kDa
    Observed band size : 240 kDa (why is the actual band size different from the predicted?)
  • Anti-ARID2 antibody [2036C1a] (ab51019) at 1/50 dilution + F2408 whole cell lysate at 50 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 197 kDa
    Observed band size : 240 kDa (why is the actual band size different from the predicted?)

参考文献

ab51019 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for forwarding the details of your protocol. The protocol looks fine, especially since you were able to detect a different protein of a similar size. I have credited your original order.

I am sorry that this antibody did not perfor...

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Thank you for contacting us.
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I am listing so...

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