製品の概要

  • 製品名Anti-ARID1B antibody
    ARID1B 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to ARID1B
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: PPAKRHEGDM YNMQYSSQQQ EMYNQYGGSY SGPDRRPIQG QYPYPYSRER MQGPGQIQTH GIPPQMMGGP LQSSSSEGPQ QNMWAARNDM PYPYQNR, corresponding to amino acids 1364-1461 of Human ARID1B

  • ポジティブ・コントロール
    • ICC/IF: HepG2 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab57461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 236 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 1-2µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性ARID1B (AT-rich interactive domain-containing protein 1B) is a component of SWI/SNF chromatin remodeling complexes involved in transcriptional activation and repression of select genes by chromatin remodeling. It binds DNA non-specifically and alters DNA-nucleosome topology.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • 6A3 5 antibody
    • ARID 1B antibody
    • ARID domain containing protein 1B antibody
    • AT rich interactive domain 1B (SWI1 like) antibody
    • AT rich interactive domain 1B antibody
    • AT rich interactive domain containing protein 1B antibody
    • BAF 250b antibody
    • BAF-associated factor, 250-KD, B antibody
    • BAF250b antibody
    • BRG1 associated factor 250b antibody
    • BRG1 binding protein antibody
    • BRG1 binding protein ELD/OSA1 antibody
    • BRG1 binding protein hELD/OSA1 antibody
    • BRIGHT antibody
    • DAN 15 antibody
    • DAN15 antibody
    • Eld (eyelid)/Osa protein antibody
    • ELD/OSA1 antibody
    • hELD/OSA1 antibody
    • hOsa 2 antibody
    • hOsa2 antibody
    • KIAA1235 antibody
    • MRD12 antibody
    • OSA 2 antibody
    • Osa homolog 2 antibody
    • OSA2 antibody
    • OTTHUMP00000040115 antibody
    • p250R antibody
    • RP11 419L10.1 antibody
    see all

Anti-ARID1B antibody 画像

  • ICC/IF image of ab57461 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57461, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ARID1B antibody (ab57461) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human kidney.


  • Predicted band size : 236 kDa
    ARID1B antibody (ab57461) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HEK293 cells stained with ab57461 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57461, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-ARID1B antibody (ab57461) 使用論文

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA pH8.5
Permeabilization No
Specification kidney
Blocking step Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative Formaldehyde
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投稿 Sep 07 2015

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (JHH4)
Loading amount 10 µg
Specification JHH4
Treatment Use nuclear fraction or cytoplasmic fraction
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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投稿 Dec 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"