製品の概要

  • 製品名
    Anti-ARHI antibody
    ARHI 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ARHI
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant protein of Human ARHI (NP_004666.1).

  • ポジティブ･コントロール
    • WB: WiDr cell line lysates IHC-P: Human brain tissue

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項（精製）
    ab107051 is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab107051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 26 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 組織特異性
    Expressed in normal ovarian and breast epithelial cells but not in ovarian and breast cancers.
  • 配列類似性
    Belongs to the small GTPase superfamily. Di-Ras family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • DIRA3 antibody
      • DIRA3_HUMAN antibody
      • DIRAS family GTP binding RAS like 3 antibody
      • DIRAS family GTPase 3 antibody
      • DIRAS family, GTP-binding RAS-like protein 3 antibody
      • DIRAS3 antibody
      • Distinct subgroup of the Ras family member 3 antibody
      • GTP binding protein Di Ras3 antibody
      • GTP-binding protein Di-Ras3 antibody
      • NOEY2 antibody
      • Ras homolog gene family member I antibody
      • Rho related GTP binding protein RhoI antibody
      • Rho-related GTP-binding protein RhoI antibody
      • RHOI antibody
      see all

    画像

    • Flow Cytometry - Anti-ARHI antibody (ab107051)
      Flow Cytometry - Anti-ARHI antibody (ab107051)

      ab107051 flow cytometric analysis of WiDr cells (right histogram) compared to a hegative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

    • Western blot - ARHI antibody (ab107051)
      Western blot - ARHI antibody (ab107051)
      Anti-ARHI antibody (ab107051) at 1/100 dilution + WiDr cell line lysates at 35 µg

      Predicted band size: 26 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ARHI antibody (ab107051)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ARHI antibody (ab107051)
      ab107051, at a1/50 dilution, staining ARHI in formalin fixed and paraffin embedded Human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

    参考文献

    This product has been referenced in:
    • Ouyang J  et al. The role of aplysia ras homolog I in colon cancer cell invasion and adhesion. Exp Ther Med 14:5193-5199 (2017). Read more (PubMed: 29201236) »
    • Li LL  et al. JMJD2A contributes to breast cancer progression through transcriptional repression of the tumor suppressor ARHI. Breast Cancer Res 16:R56 (2014). Read more (PubMed: 24886710) »

    See all 2 Publications for this product

    ab107051 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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