製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ARHGEF1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 900 to the C-terminus of Mouse ARHGEF1.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in NIH3T3 whole cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab93447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).

ターゲット情報

  • 機能
    Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.
  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 RGSL (RGS-like) domain.
  • ドメイン
    The RGSL domain, also known as rgRGS domain, is necessary but not sufficient for GAP activity.
    The DH domain is involved in interaction with CCPG1.
  • 翻訳後修飾
    Phosphorylated by PKCA. Angiotensin-2 induced Tyr-738 phosphorylation is mediated by JAK2.
  • 細胞内局在
    Cytoplasm. Membrane. Translocated to the membrane by activated GNA13 or LPA stimulation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 115 kD protein antibody
    • 115 kDa guanine nucleotide exchange factor antibody
    • ARHG1_HUMAN antibody
    • ARHGEF 1 antibody
    • ARHGEF1 antibody
    • GEF 1 antibody
    • GEF1 antibody
    • LBCL 2 antibody
    • LBCL2 antibody
    • LSC antibody
    • Lsc homolog antibody
    • p115 RhoGEF antibody
    • p115-RhoGEF antibody
    • p115RhoGEF antibody
    • Rho guanine nucleotide exchange factor (GEF) 1 antibody
    • Rho guanine nucleotide exchange factor 1 antibody
    • Sub1.5 antibody
    see all

画像

  • Anti-ARHGEF1 antibody (ab93447) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 102 kDa
    Observed band size : 102 kDa


    Exposure time : 20 minutes

プロトコール

参考文献

ab93447 has not yet been referenced specifically in any publications.

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