製品の概要

  • 製品名
    Anti-ARHGAP31 antibody
  • 製品の詳細
    Rabbit polyclonal to ARHGAP31
  • アプリケーション
    適用あり: ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence, corresponding to amino acids 1163-1261 (QDLDIVAHAL TGRRNSAPVS VSAVRTSFMV KMCQARAVPV IPPKIQYTQI PQPLPSQSSG ENGVQPLERS QEGPSSTSGT TQKPAKDDSP SSLESSKEE) of Human ARHGAP31.

  • ポジティブ・コントロール
    • Human placenta tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab122331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Functions as a GTPase-activating protein (GAP) for RAC1 and CDC42. Required for cell spreading, polarized lamellipodia formation and cell migration.
  • 配列類似性
    Contains 1 Rho-GAP domain.
  • 発生段階
    Mainly expressed in fetal heart and muscle.
  • 翻訳後修飾
    Phosphorylation on Thr-789 reduces GAP activity.
  • 細胞内局在
    Cell projection > lamellipodium. Cell junction > focal adhesion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ARHGAP31 antibody
    • Cdc42 GTPase-activating protein antibody
    • RHG31_HUMAN antibody
    • Rho GTPase-activating protein 31 antibody

画像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, cytoplasm and golgi apparatus. Recommended concentration of ab122331 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122331, at 1/300 dilution, staining ARHGAP31 in paraffin-embedded Human placenta tissue, by Immunohistochemistry.

参考文献

ab122331 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab122331.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録