Rabbit polyclonal to ARHGAP31
antigen sequence, corresponding to amino acids 1163-1261 (QDLDIVAHAL TGRRNSAPVS VSAVRTSFMV KMCQARAVPV IPPKIQYTQI PQPLPSQSSG ENGVQPLERS QEGPSSTSGT TQKPAKDDSP SSLESSKEE) of Human ARHGAP31.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Functions as a GTPase-activating protein (GAP) for RAC1 and CDC42. Required for cell spreading, polarized lamellipodia formation and cell migration.
Contains 1 Rho-GAP domain.
Mainly expressed in fetal heart and muscle.
Phosphorylation on Thr-789 reduces GAP activity.
Cell projection > lamellipodium. Cell junction > focal adhesion.
Information by UniProt
Cdc42 GTPase-activating protein antibody
Rho GTPase-activating protein 31 antibody
Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP31 antibody (ab122331)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, cytoplasm and golgi apparatus. Recommended concentration of ab122331 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARHGAP31 antibody (ab122331)
ab122331, at 1/300 dilution, staining ARHGAP31 in paraffin-embedded Human placenta tissue, by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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