製品の概要

  • 製品名
    Anti-ARHGAP23 antibody
  • 製品の詳細
    Rabbit polyclonal to ARHGAP23
  • アプリケーション
    適用あり: ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence: MNLGFGDESP EPEASGRGER LGRKVAPLAT TEDSLASIPF IDEPTSPSID LQAKHVPASA VVSSAMNSAP VLGTSPSS, corresponding to amino acids 509-586 of Human ARHGAP23.

  • ポジティブ・コントロール
    • Human cerebellum tissue; RT 4 and U 251 MG lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab121617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.
  • 組織特異性
    Expressed in placenta, prostate, hippocampus and brain medulla. Also expressed in brain tumor, salivary gland tumor, head and neck tumor.
  • 配列類似性
    Contains 1 PDZ (DHR) domain.
    Contains 1 PH domain.
    Contains 1 Rho-GAP domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Arhgap23 antibody
    • KIAA1501 antibody
    • RHG23_HUMAN antibody
    • Rho GTPase-activating protein 23 antibody
    • Rho-type GTPase-activating protein 23 antibody
    see all

画像

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and plasma membrane. Recommended concentration of ab121617 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121617 at 1/125 staining ARHGAP23 in Paraffin Embedded Human cerebellum tissue by Immunohistochemistry.

参考文献

ab121617 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat Hippocampal Neurons)
Permeabilization
Yes - 0.2% Triton-X 100 in PBS
Specification
Rat Hippocampal Neurons
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Formaldehyde
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投稿 Mar 13 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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