製品の概要

  • 製品名Anti-ARHGAP11A antibody
    ARHGAP11A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ARHGAP11A
  • アプリケーション適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between residue 973 and 1023 of Human ARHGAP11A using the numbering given in entry NP_055598.1 (GeneID 9824).

  • ポジティブ・コントロール
    • HeLa, 293T or NIH3T3 cell lysate. Human skin FFPE tissue sections.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH range: 7 - 8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab113261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 114 kDa.
IP Use at 2-10 µg/mg of lysate.

ターゲット情報

Anti-ARHGAP11A antibody 画像

  • All lanes : Anti-ARHGAP11A antibody (ab113261) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 114 kDa


    Exposure time : 3 minutes
  • Lane 1: 1mg whole cell lysate from HeLa cells was immunoprecipitated with ab113261 at 6µg/mg lysate and 20% IP was loaded on gel.
    Lane 2: same, with a control IgG.
    For WB ab113261 was used at 1µg/ml.
    Detection: chemiluminescence with exposure times of 10 seconds.
  • IHC image of ARHGAP11A staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113261, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-ARHGAP11A antibody (ab113261) 使用論文

ab113261 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"