製品の概要

  • 製品名Anti-Argonaute 5 antibody
  • 製品の詳細
    Rabbit polyclonal to Argonaute 5
  • 特異性ab4723 detects a band of ~ 75 kDa in P19 embryonic carcinoma lysate. The predicted molecular weight of murine Argonaute 5 is 72 kDa according to Swissprot entry Q8CGT7. (This represents a partial sequence) Other bands of 85, 160 and 250 kDa are also detected which may be non-specific binding or alternatively may correspond to the full length Argonaute 5.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse Argonaute 5.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in F9 whole cell lysate.
  • 特記事項Swiss prot Q8CGT7 - fragment

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab4723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).

ターゲット情報

  • 関連性Argonaute proteins make up a highly conserved family whose members have been implicated in RNAi and related phenomena in several organisms. In addition to roles in RNAi-like mechanisms, Argonaute proteins influence development, and at least a subset are involved in stem cell fate determination. Argonaute proteins are ~100-kD highly basic proteins that contain two common domains, namely PAZ and PIWI domains (Cerutti et al. 2000). The PAZ domain, consisting of 130 amino acids, has been identified in Argonaute proteins and in Dicer (Bernstein et al. 2001a). Although it has no defined function, PAZ is thought to be a protein-protein interaction domain, potentially mediating both homo- and heterodimerization (Cerutti et al. 2000). There are several reports that Argonaute proteins, being highly basic, might bind RNA. Miwi, a murine homolog of Piwi, exists in a complex with its target mRNAs in vivo (Deng and Lin 2002). Furthermore, Drosophila Ago1 has been shown to bind ribohomopolymers in vitro (Kataoka et al. 2001). Of course, the involvement of Argonaute proteins in RNAi and related phenomena presupposes an interaction with RNA, either directly or indirectly. However, detailed studies of Argonaute structure and biochemistry will be required to reveal its precise function in RNA metabolism.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • Ago 5 antibody
    • Ago5 antibody
    • Argonaute 5 protein antibody
    • Argonaute5 antibody
    • Eif2c5 antibody
    • Eukaryotic translation initiation factor 2C 5 antibody
    see all

Anti-Argonaute 5 antibody 画像



  • Predicted band size : 72 kDa

    This image was submitted as part or a review by Aarti Jagannath

    30µg of p19 murine embryonic carcinoma cell lysate was run on an SDS-PAGE and incubated for 12 hours with ab4723 (1/1000 dilution). A specific band of ~ 75 kDa is detected.

     

  • Anti-Argonaute 5 antibody (ab4723) at 1 µg/ml + F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 72 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

Anti-Argonaute 5 antibody (ab4723) 使用論文

ab4723 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (P19 embryonic carcinoma cell line)
Loading amount 30 µg
Specification P19 embryonic carcinoma cell line
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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投稿 May 26 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"