Customer reviews and FAQs

製品の概要

  • 製品名Anti-Argonaute 4 antibody
  • 製品の詳細
    Rabbit polyclonal to Argonaute 4
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    A 15 amino acid synthetic peptide (Human) from near the N-terminus

  • ポジティブ･コントロール
    • HeLa cell lysate

法規制情報

医薬用外毒物

製品の特性

  • 製品の状態Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab85077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250 - 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
IHC-P 1/200.

ターゲット情報

  • 機能Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for RNA-directed transcription and replication of the human hapatitis delta virus (HDV).
  • 配列類似性Belongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • 細胞内局在Cytoplasm > P-body.
  • Information by UniProt
  • 参照データベース
    • 別名
      • 5730550L01Rik antibody
      • Ago 4 antibody
      • Ago4 antibody
      • AGO4_HUMAN antibody
      • Argonaute4 antibody
      • EIF 2C 4 antibody
      • eIF-2C 4 antibody
      • eIF2C 4 antibody
      • eif2c4 antibody
      • Eukaryotic translation initiation factor 2C 4 antibody
      • eukaryotic translation initiation factor 2C antibody
      • hAgo4 antibody
      • KIAA1567 antibody
      • Piwi/Argonaute family protein meIF2C4 antibody
      • Protein argonaute-4 antibody
      see all

    Anti-Argonaute 4 antibody 画像

    • Western blot - Argonaute 4 antibody (ab85077)
      Western blot - Argonaute 4 antibody (ab85077)
      Anti-Argonaute 4 antibody (ab85077) at 1/500 dilution + HeLa cell lysate

      Predicted band size : 97 kDa
      Observed band size : 97 kDa
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute 4 antibody (ab85077)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute 4 antibody (ab85077)This image is courtesy of an anonymous Abreview
      ab85077 staining Argonaute 4 in Mouse testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/200 in PBS + casein) for 1 hour 30 mins at 37°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

      See Abreview

    Anti-Argonaute 4 antibody (ab85077) 使用論文

    This product has been referenced in:
    • Zhong F  et al. A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes. Nucleic Acids Res 43:10474-91 (2015). WB . Read more (PubMed: 26405199) »

    See 1 Publication for this product

    ab85077 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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    ab85077 の abreview を投稿する ab85077 に関する問い合わせをする

    3 Abreviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Argonaute 4 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (testis)
    Specification testis
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated
    Permeabilization No
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More
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    Abcam user community

    Verified customer

    投稿 Apr 11 2012

    CLIP abreview for Anti-Argonaute 4 antibody

     Inconclusive
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application CLIP
    Sample Human Cell lysate - whole cell (HEK 293T)
    Specification HEK 293T
    Type iCLIP
    Irradiation Energy Level (mJ/cm2): 150
    Wavelength (nm): 254
    Positive control Anti-hnRNP C antibody (sc-15386) bound protein A dynabeads, 0.1 mg/ml (antibody/beads)
    Negative control Protein A dynabeads without antibody bound
    Immuno-precipitation step Other - Protein A dynabeads
    Read More
    Username

    Abcam user community

    Verified customer

    投稿 Mar 21 2012

    Hi，colleague     I have noticed that there is one product anti-AGO4 used for WB assays in abcam.Then I'd like to make sure  if this product  is useful for co-immunoprecipitation or do you have  other antibodies such as anti-AGO2 designed for co-IP assa...

    Read More

    Thank you for your enquiry. To date, neither of our anti-AGO4 antibodies have been tested in IP. Both ab85077 and ab52724 have thusfar only been tested in western blotting. They may be useful for IP but we do not have any data to suggest it. ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"