製品の概要
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製品名Anti-Argonaute 4 antibody
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製品の詳細Rabbit polyclonal to Argonaute 4
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由来種Rabbit
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アプリケーション適用あり: WB, IHC-Pmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
A 15 amino acid synthetic peptide (Human) from near the N-terminus
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ポジティブ･コントロール
- HeLa cell lysate
法規制情報
製品の特性
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製品の状態Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
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保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA -
Concentration information loading... -
精製度Protein A purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab85077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/250 - 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa). | |
| IHC-P | 1/200. |
ターゲット情報
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機能Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for RNA-directed transcription and replication of the human hapatitis delta virus (HDV).
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配列類似性Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
細胞内局在Cytoplasm > P-body.
- Information by UniProt
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参照データベース
- Entrez Gene: 192670 Human
- Entrez Gene: 76850 Mouse
- Entrez Gene: 298533 Rat
- Omim: 607356 Human
- SwissProt: Q9HCK5 Human
- SwissProt: Q8CJF8 Mouse
- Unigene: 471492 Human
- Unigene: 86705 Mouse
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別名
- 5730550L01Rik antibody
- Ago 4 antibody
- Ago4 antibody
see all
画像
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Western blot - Argonaute 4 antibody (ab85077)Anti-Argonaute 4 antibody (ab85077) at 1/500 dilution + HeLa cell lysate
Predicted band size: 97 kDa
Observed band size: 97 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute 4 antibody (ab85077)This image is courtesy of an anonymous Abreviewab85077 staining Argonaute 4 in Mouse testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/200 in PBS + casein) for 1 hour 30 mins at 37°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
参考文献
This product has been referenced in:
- Yu D et al. Multiple microRNAs function as self-protective modules in acetaminophen-induced hepatotoxicity in humans. Arch Toxicol 92:845-858 (2018). EMSA ; Human . Read more (PubMed: 29067470) »
- Voutila J et al. Development and Mechanism of Small Activating RNA Targeting CEBPA, a Novel Therapeutic in Clinical Trials for Liver Cancer. Mol Ther 25:2705-2714 (2017). Read more (PubMed: 28882451) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"