製品の概要

  • 製品名Anti-Argonaute 3 / eIF2C 3 antibody
    Argonaute 3 / eIF2C 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Argonaute 3 / eIF2C 3
  • アプリケーション適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide derived from within residues 350 - 450 of Human Argonaute 3.

    (Peptide available as ab24437.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in rat and mouse dorsal root ganglia tissue lysates in WB.
  • 特記事項Protein: NP_079128 (human isoform a); NP_803171 (human isoform b); NP_700451 (mouse).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
ICC/IF 1/200.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs.
  • 配列類似性Belongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • 細胞内局在Cytoplasm > P-body.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5730550L01Rik antibody
    • Ago 3 antibody
    • Ago3 antibody
    • AGO3_HUMAN antibody
    • argonaute 3 antibody
    • Argonaute3 antibody
    • eIF 2C 3 antibody
    • eIF-2C 3 antibody
    • eIF2C 3 antibody
    • Eif2c3 antibody
    • EIF2C3 protein antibody
    • Eukaryotic translation initiation factor 2C 3 antibody
    • Eukaryotic translation initiation factor 2C3 antibody
    • FLJ12765 antibody
    • hAgo3 antibody
    • MGC86946 antibody
    • Protein argonaute-3 antibody
    see all

Anti-Argonaute 3 / eIF2C 3 antibody 画像

  • Rat dorsal root ganglia were fixed in paraformaldehyde, blocked with 2% BSA for 30 minutes then incubated with ab3593 (1/200 dilution). For further details please refer to Hengst U et al  (2006).

  • All lanes : Anti-Argonaute 3 / eIF2C 3 antibody (ab3593) at 2 µg/ml

    Lane 1 : Dorsal Root Ganglia (Rat) Tissue Lysate
    Lane 2 : Dorsal Root Ganglia (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 97 kDa
    Observed band size : 97 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • ab3593 staining Argonaute 3 / eIF2C 3 in murine testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed with formaldehyde and an enzymatic antigen retrieval step was performed. Samples were then blocked with 10% serum for 30 minutes at 25°C and then incubated with ab3593 at a 1/50 dilution for 1 hour 30 minutes at 37°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.

    See Abreview

Anti-Argonaute 3 / eIF2C 3 antibody (ab3593) 使用論文

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application CLIP
Sample Human Cell lysate - whole cell (HEK 293T)
Specification HEK 293T
Type iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control Anti-hnRNP C antibody (sc-15386) bound protein A dynabeads, 0.1 mg/ml (antibody/beads)
Negative control Protein A dynabeads without antibody bound
Immuno-precipitation step Other - Protein A dynabeads
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Abcam user community

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投稿 Mar 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (testis)
Specification testis
Fixative Formaldehyde
Antigen retrieval step Enzymatic
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
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Dr. Lynn Dong

Verified customer

投稿 Feb 28 2012

Application Western blot
Sample Human Cell lysate - whole cell (human umbilical vein endothelial cells)
Loading amount 50 µg
Specification human umbilical vein endothelial cells
Gel Running Conditions Reduced Denaturing (SDS-PAGE 8%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

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投稿 Mar 31 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (dissociated dorsal root ganglia)
Specification dissociated dorsal root ganglia
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2%
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Abcam user community

Verified customer

投稿 Jun 07 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (dorsal root ganglia explants)
Loading amount 50 µg
Specification dorsal root ganglia explants
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
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Abcam user community

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投稿 Apr 28 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"