製品の概要

  • 製品名Anti-Arginyl tRNA synthetase antibody
    Arginyl tRNA synthetase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Arginyl tRNA synthetase
  • 特異性This antibody reacts specifically with 74kDa RRS and immunoprecipitates with AIMP1(p43).
  • アプリケーション適用あり: IHC-P, WB, ICC/IF, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Native recombinant corresponding to amino acids 1-72 of Human RRS.

  • ポジティブ・コントロール
    • HEK293 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab31537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100.
WB 1/3000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
ICC/IF 1/4000 - 1/8000.
IP 1/4000 - 1/8000.

ターゲット情報

  • 機能Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1.
  • 配列類似性Belongs to the class-I aminoacyl-tRNA synthetase family.
  • ドメインThe N-terminus (AA 1-72) has two regions predicted to be alpha-helical that might be involved in the multisynthetase complex assembly.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 製品の状態There are 2 isoforms produced by alternative initiation.
  • 別名
    • 2610011N19Rik antibody
    • 2610037E21Rik antibody
    • AL033339 antibody
    • Arginine tRNA ligase 1, cytoplasmic antibody
    • Arginine tRNA ligase antibody
    • Arginine--tRNA ligase antibody
    • Arginyl tRNA synthetase 1 antibody
    • Arginyl tRNA synthetase cytoplasmic antibody
    • Arginyl tRNA synthetase, cytoplasmic antibody
    • Arginyl-tRNA synthetase, cytoplasmic antibody
    • ArgRS antibody
    • AW985894 antibody
    • DALRD 1 antibody
    • DALRD1 antibody
    • EC 6.1.1.19 antibody
    • MGC8641 antibody
    • MGC86730 antibody
    • RARS antibody
    • SYRC_HUMAN antibody
    • wu:fa96g09 antibody
    • zgc:56050 antibody
    • zgc:86730 antibody
    see all

Anti-Arginyl tRNA synthetase antibody 画像

  • Anti-Arginyl tRNA synthetase antibody (ab31537) at 1/4000 dilution + HEK293 cell lysate

    Predicted band size : 74 kDa
    Observed band size : 74 kDa
  • Immunofluorescence of a Chang cell using ab31537 at a dilution of 1/1000.
  • Formalin fixed-paraffin embedded Immunohistochemistry staining of Human breast tissue using ab31537 at 1/4000.
  • Anti-Arginyl tRNA synthetase antibody (ab31537) at 1/3000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml

    Secondary
    Goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 74 kDa
    Observed band size : 74 kDa

Anti-Arginyl tRNA synthetase antibody (ab31537) 使用論文

This product has been referenced in:
  • Park SJ  et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One 10:e0142253 (2015). WB ; Human . Read more (PubMed: 26544075) »
  • Zhang X  et al. Mutations in QARS, encoding glutaminyl-tRNA synthetase, cause progressive microcephaly, cerebral-cerebellar atrophy, and intractable seizures. Am J Hum Genet 94:547-58 (2014). Read more (PubMed: 24656866) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hepatocarcinoma)
Loading amount 100 µg
Specification Hepatocarcinoma
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. Silvia Marquez Jurado

Verified customer

投稿 Sep 26 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Pig Cell (Swine Cell)
Specification Swine Cell
Fixative Formaldehyde
Permeabilization Yes - Triton-100 05% in PBS 1X
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Ms. Silvia Marquez Jurado

Verified customer

投稿 Sep 14 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"