製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Arg 3.1
  • アプリケーション
    適用あり: IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat Arg3.1.

    (Peptide available as ab26899.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Mouse forebrain, Mouse hippocampus, Rat cortex, Rat hippocampus.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab23382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/300.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 45 kDa).

Abcam recommends using 3% milk as the blocking agent.

ターゲット情報

  • 機能
    Required for consolidation of synaptic plasticity as well as formation of long-term memory. Regulates endocytosis of AMPA receptors in response to synaptic activity. Required for homeostatic synaptic scaling of AMPA receptors (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration.
  • 配列類似性
    Belongs to the ARC/ARG3.1 family.
  • 細胞内局在
    Cytoplasm, cytoskeleton. Endosome. Cytoplasmic vesicle, secretory vesicle, acrosome. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, dendrite. Cell projection, dendritic spine. Cell junction, synapse. Associated with the cell cortex of neuronal soma and dendrites. Enriched in postsynaptic density of dendritic spines. Associated with the sperm tail (By similarity). Enriched on the plasma membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activity regulated cytoskeletal associated protein antibody
    • Activity regulated cytoskeletal protein antibody
    • Activity regulated gene 3.1 protein homolog antibody
    • Activity-regulated cytoskeleton-associated protein antibody
    • Activity-regulated gene 3.1 protein homolog antibody
    • Arc antibody
    • ARC/ARG3.1 antibody
    • ARC_HUMAN antibody
    • Arg3.1 antibody
    • KIAA0278 antibody
    see all

Anti-Arg 3.1 antibody 画像

  • All lanes : Anti-Arg 3.1 antibody (ab23382) at 1 µg/ml (with 3% milk block)

    Lane 1 : Forebrain (Mouse) Tissue Lysate
    Lane 2 : Rat Cortex Tissue Lysate
    Lane 3 : Hippocampus (Mouse) Tissue Lysate
    Lane 4 : Rat Hippocampus Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 12 minutes
  • All lanes : Anti-Arg 3.1 antibody (ab23382) at 1 µg/ml

    Lane 1 : Hippocampus (Mouse) Tissue Lysate blocked with 5% BSA
    Lane 2 : Rat Hippocampus Tissue Lysate blocked with 5% BSA
    Lane 3 : Hippocampus (Mouse) Tissue Lysate blocked with 5% milk
    Lane 4 : Rat Hippocampus Tissue Lysate blocked with 5% milk
    Lane 5 : Hippocampus (Mouse) Tissue Lysate blocked with 3% milk
    Lane 6 : Rat Hippocampus Tissue Lysate blocked with 3% milk
    Lane 7 : Hippocampus (Mouse) Tissue Lysate blocked with 1% milk
    Lane 8 : Rat Hippocampus Tissue Lysate blocked with 1% milk

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

Anti-Arg 3.1 antibody (ab23382) 使用論文

This product has been referenced in:
  • Wu H  et al. Salicylate-induced changes in immediate-early genes in the hippocampal CA1 area. Mol Med Rep 12:1625-30 (2015). WB, IHC-P ; Rat . Read more (PubMed: 25873216) »

See 1 Publication for this product

Product Wall

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8% Bis-Tris in MES)
Sample
Mouse Tissue lysate - other (Hippocampal Cytoplasm)
Specification
Hippocampal Cytoplasm
Blocking step
Li-Cor Block Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
Username

Grant Corbett

Verified customer

投稿 Jul 23 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Spinal cord)
Specification
Spinal cord
Fixative
Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

投稿 Dec 10 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (e14.5 striatum)
Specification
e14.5 striatum
Fixative
Paraformaldehyde
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Dean Griffiths

Verified customer

投稿 Aug 17 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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