Anti-ARFGEF2 抗体 (ab72420)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ARFGEF2
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 300 and 350 of human ARFGEF2 (NP_006411.1)

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 202 kDa (predicted molecular weight: 202 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性
    ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. ARFGEF2 promotes guanine-nucleotide exchange on ARF1, ARF5 and ARF6 and the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition.
  • 細胞内局在
    Cytoplasm. Membrane. Golgi apparatus. Cytoplasm › perinuclear region. Golgi apparatus › trans-Golgi network By similarity. Endosome By similarity. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Cell projection › dendrite By similarity. Cytoplasmic vesicle By similarity. Cell junction › synapse By similarity. Cytoplasm › cytoskeleton By similarity. Note: Translocates from cytoplasm to membranes upon cAMP treatment. Localized in recycling endosomes.
  • 参照データベース
  • 別名
    • ADP ribosylation factor guanine nucleotide exchange factor 2 (brefeldin A inhibited) antibody
    • ADP ribosylation factor guanine nucleotide exchange factor 2 antibody
    • ARFGEF 2 antibody
    • ARFGEF2 antibody
    • ARFGEP2 antibody
    • BIG 2 antibody
    • BIG2 antibody
    • Brefeldin A inhibited 2 antibody
    • Brefeldin A inhibited GEP 2 antibody
    • Brefeldin A inhibited guanine nucleotide exchange protein 2 antibody
    • dJ1164I10.1 antibody
    • FLJ23723 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ARFGEF2 with ab72420 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-ARFGEF2 antibody (ab72420) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg

    Developed using the ECL technique.

    Predicted band size: 202 kDa
    Observed band size: 202 kDa


    Exposure time: 3 minutes
  • Detection of ARFGEF2 by Western Blot of Immunprecipitate.
    ab72420 at 1µg/ml staining ARFGEF2 in HeLa whole cell lysate immunoprecipitated using ab72420 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

参考文献

ab72420 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Vascular Smooth Muscle Cells)
Gel Running Conditions
Reduced Denaturing (4-20% Gradient)
Loading amount
10 µg
Specification
Vascular Smooth Muscle Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 May 24 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Vascular Smooth Muscle Cells)
Antigen retrieval step
None
Permeabilization
Yes - PBS+0.25% Triton X-100 (10 mins)
Specification
Vascular Smooth Muscle Cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Michael Morran

Verified customer

投稿 May 18 2018

We testedab72420 for reactivity to mouse protein and found it did not react.

I hope this is helpful. Please contact me again if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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