製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ARFGAP3
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 466 and 516 of human ARFGAP3 (NP_055385.2)

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能
    GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.
  • 組織特異性
    Widely expressed. Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary.
  • 配列類似性
    Contains 1 Arf-GAP domain.
  • 発生段階
    Expressed at higher level in adult thymus, brain and lung, than in corresponding fetal tissues. Expressed at lower level in spleen, heart, kidney and liver during development.
  • 細胞内局在
    Cytoplasm. Golgi apparatus membrane. Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADP ribosylation factor GTPase activating protein 1 antibody
    • ADP ribosylation factor GTPase activating protein 3 antibody
    • ADP-ribosylation factor GTPase-activating protein 3 antibody
    • ARF GAP 3 antibody
    • ARFG3_HUMAN antibody
    • ArfGAP 3 antibody
    • ARFGAP1 antibody
    • ARFGAP3 antibody
    • FLJ45618 antibody
    see all

画像

  • All lanes : Anti-ARFGAP3 antibody (ab85971) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 57 kDa
    Observed band size : 63 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Detection of ARFGAP3 by Western Blot of Immunprecipitate.
    ab85971 at 1µg/ml staining ARFGAP3 in HeLa whole cell lysate immunoprecipitated using ab85971 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

参考文献

This product has been referenced in:
  • Anthony DF  et al. {beta}-Arrestin 1 Inhibits the GTPase-Activating Protein Function of ARHGAP21, Promoting Activation of RhoA following Angiotensin II Type 1A Receptor Stimulation. Mol Cell Biol 31:1066-75 (2011). WB ; Human . Read more (PubMed: 21173159) »

See 1 Publication for this product

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