Anti-ARFGAP1 抗体 (ab85971)
Key features and details
- Rabbit polyclonal to ARFGAP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-ARFGAP1 antibody -
製品の詳細
Rabbit polyclonal to ARFGAP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human ARFGAP1 aa 466-516.
Database link: NP_055385.2 -
ポジティブ・コントロール
- HeLa whole cell lysate.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
ab85971 was affinity purified using an epitope specific to ARFGAP1 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85971の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/2000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
特記事項 |
---|
WB
1/2000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
-
機能
GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes. -
組織特異性
Widely expressed. Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary. -
配列類似性
Contains 1 Arf-GAP domain. -
発生段階
Expressed at higher level in adult thymus, brain and lung, than in corresponding fetal tissues. Expressed at lower level in spleen, heart, kidney and liver during development. -
細胞内局在
Cytoplasm. Golgi apparatus membrane. Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment. - Information by UniProt
-
参照データベース
- Entrez Gene: 458885 Chimpanzee
- Entrez Gene: 26286 Human
- Entrez Gene: 711160 Rhesus monkey
- Omim: 612439 Human
- SwissProt: Q9NP61 Human
- SwissProt: Q5R787 Orangutan
- Unigene: 685225 Human
-
別名
- ADP ribosylation factor GTPase activating protein 1 antibody
- ADP ribosylation factor GTPase activating protein 3 antibody
- ADP-ribosylation factor GTPase-activating protein 3 antibody
see all
画像
-
All lanes : Anti-ARFGAP1 antibody (ab85971) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 57 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Detection of ARFGAP1 by Western Blot of Immunprecipitate.
ab85971 at 1µg/ml staining ARFGAP1 in HeLa whole cell lysate immunoprecipitated using ab85971 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab85971 は 1 報の論文で使用されています。
- Anthony DF et al. {beta}-Arrestin 1 Inhibits the GTPase-Activating Protein Function of ARHGAP21, Promoting Activation of RhoA following Angiotensin II Type 1A Receptor Stimulation. Mol Cell Biol 31:1066-75 (2011). WB ; Human . PubMed: 21173159