• 製品名
  • 製品の詳細
    Rabbit polyclonal to ARFBP1
  • アプリケーション
    適用あり: ICC/IF, WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human ARFBP1 aa 2250-2300.
    Database link: Q7Z6Z7

  • ポジティブ・コントロール
    • Whole cell lysate from 293T cells.


  • 製品の状態
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
  • アイソタイプ
  • 研究分野


Our Abpromise guarantee covers the use of ab70161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000 - 1/5000. Detects a band of approximately >460 kDa (predicted molecular weight: 481 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.


  • 機能
    E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation.
  • 組織特異性
    Weakly expressed in heart, brain and placenta but not in other tissues. Expressed in a number of cell lines, predominantly in those from colorectal carcinomas.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 関連疾患
    Defects in HUWE1 are the cause of mental retardation syndromic X-linked Turner type (MRXST) [MIM:300706]; also known as mental retardation and macrocephaly syndrome. MRXST shows clinical variability. Associated phenotypes include macrocephaly and variable contractures.
    A chromosomal microduplication involving HUWE1 and HSD17B10 is the cause of mental retardation X-linked type 17 (MRX17) [MIM:300705]; also known as mental retardation X-linked type 31 (MRX31). Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific X-linked mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic X-linked mental retardation.
  • 配列類似性
    Belongs to the TOM1/PTR1 family.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 1 UBA domain.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
    Contains 1 WWE domain.
  • ドメイン
    The HECT domain mediates inhibition of the transcriptional activity of p53.
  • 翻訳後修飾
    Phosphorylated on tyrosine; phosphorylation is probably required for its ability to inhibit TP53 transactivation.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Cytoplasm. Nucleus. Mainly expressed in the cytoplasm of most tissues, except in the nucleus of spermatogonia, primary spermatocytes and neuronal cells (By similarity). Predominantly cytosolic or perinuclear in some colorectal carcinoma cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ARF binding protein 1 antibody
    • ARF BP1 antibody
    • ARF-binding protein 1 antibody
    • ARF-BP1 antibody
    • BJ-HCC-24 tumor antigen antibody
    • E3 ubiquitin protein ligase HUWE1 antibody
    • E3 ubiquitin-protein ligase HUWE1 antibody
    • HECT antibody
    • HECT domain protein LASU1 antibody
    • HECT UBA and WWE domain containing protein 1 antibody
    • HectH9 antibody
    • Homologous to E6AP carboxyl terminus homologous protein 9 antibody
    • Huwe1 antibody
    • HUWE1_HUMAN antibody
    • Ib772 antibody
    • Large structure of UREB1 antibody
    • LASU1 antibody
    • Mcl 1 ubiquitin ligase E3 antibody
    • Mcl-1 ubiquitin ligase E3 antibody
    • Mule antibody
    • UBA and WWE domain-containing protein 1 antibody
    • Upstream regulatory element-binding protein 1 antibody
    • URE-B1 antibody
    • URE-binding protein 1 antibody
    • UREB1 antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling ARFBP1 with ab70161 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-ARFBP1 antibody (ab70161) at 1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 5 µg

    Predicted band size : 481 kDa
    Observed band size : >460 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa,300 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab70161 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70161, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab70161 staining in human normal heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab70161, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:
  • Park SH  et al. Metformin enhances TRAIL-induced apoptosis by Mcl-1 degradation via Mule in colorectal cancer cells. Oncotarget 7:59503-59518 (2016). Read more (PubMed: 27517746) »
  • Horikawa I  et al. Autophagic degradation of the inhibitory p53 isoform ?133p53a as a regulatory mechanism for p53-mediated senescence. Nat Commun 5:4706 (2014). Read more (PubMed: 25144556) »

See all 4 Publications for this product

レビューと Q&A

Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10%)
Human Cell lysate - whole cell (HEK293)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Jan 29 2014

Thank you for your email.
I have added a vial of ab70161 in Order 1086585. It wasn't possible to add the vial in the current "dispatch today" order.
I hope this informaiton will be helpful.
Have a good weekend!

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital info...

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Thank you for your email. I am sorry to hear that you are experiencing problems with this antibody.
I have checked the protocol and images you have kindly sent I however have few following questions:
- The molecular weight of ARFBP1 is ˜400 k...

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