The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 5 µg/ml.
Use at an assay dependent concentration.
ELISA titre using peptide based assay, 1:62500.
GTP-binding protein involved in protein trafficking; regulates endocytic recycling and cytoskeleton remodeling. May modulate vesicle budding and uncoating within the Golgi apparatus. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in the regulation of dendritic spine development (By similarity). Contributes to the regulation of dendritic branching and filopodia extension.
Belongs to the small GTPase superfamily. Arf family.
Golgi apparatus. Cell membrane. Endosome membrane. Cell membrane. Cell projection > filopodium membrane. Recruited to the cell membrane in association with CYTH2 and ARL4C.
Humphreys D et al. Arf6 coordinates actin assembly through the WAVE complex, a mechanism usurped by Salmonella to invade host cells. Proc Natl Acad Sci U S A110:16880-5 (2013).
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