製品の概要

  • 製品名Anti-ARF5 antibody
    ARF5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ARF5
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 49-113 of Human ARF5

  • ポジティブ・コントロール
    • Molt-4, Raji cells lysate

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 21 kDa.

ターゲット情報

  • 機能GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.
  • 配列類似性Belongs to the small GTPase superfamily. Arf family.
  • 細胞内局在Golgi apparatus. Cytoplasm > perinuclear region.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADP ribosylation factor 5 antibody
    • ADP-ribosylation factor 5 antibody
    • ARF 5 antibody
    • Arf5 antibody
    • ARF5_HUMAN antibody
    see all

Anti-ARF5 antibody 画像

  • All lanes : Anti-ARF5 antibody (ab96389) at 1/1500 dilution

    Lane 1 : Molt-4 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 21 kDa

Anti-ARF5 antibody (ab96389) 使用論文

ab96389 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (kidney, epithelial cells)
Loading amount 100000 cells
Specification kidney, epithelial cells
Treatment transfected (or not) with ARF5-HA and infected (or not) with influenza A virus
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 18°C
Username

Dr. Maria Amorim

Verified customer

投稿 Apr 05 2012

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (kidney, epithelial cells)
Specification kidney, epithelial cells
Fixative Formaldehyde
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 18°C
Username

Dr. Maria Amorim

Verified customer

投稿 Apr 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"