Anti-ARF1 + ARF3 抗体 [EPR443] (ab108347)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR443] to ARF1 + ARF3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ARF1 + ARF3 antibody [EPR443]
ARF1+ARF3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR443] to ARF1 + ARF3 -
由来種
Rabbit -
特異性
The immunogen used to raise this antibody shares 100% homology with human ARF3, 96% homology with ARF4 and ARF5, and 88% homology with ARF6. Cross-reactivity with the latter three proteins have not been confirmed experimentally. -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human fetal kidney, Human fetal brain and MCF7 lysates; Human breast ductal carcinoma, and Human colonic adenocarcinoma tissues This antibody gave a positive result when used in the following methanol fixed cell lines: CaCo2
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR443 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Assay kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108347の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/50 - 1/100.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/50 - 1/100. |
ターゲット情報
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細胞内局在
ARF1: Golgi apparatus. Cytoplasm > perinuclear region. ARF3: Golgi apparatus. Cytoplasm > perinuclear region. -
参照データベース
- Entrez Gene: 375 Human
- Entrez Gene: 377 Human
- Entrez Gene: 11840 Mouse
- Entrez Gene: 11842 Mouse
- Entrez Gene: 140940 Rat
- Entrez Gene: 64310 Rat
- Omim: 103180 Human
- Omim: 103190 Human
see all
画像
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All lanes : Anti-ARF1 + ARF3 antibody [EPR443] (ab108347) at 1/50000 dilution
Lane 1 : Full length human ARF-1 recombinant protein
Lane 2 : Full length human ARF-3 recombinant protein
Lysates/proteins at 0.015 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM /TBST
Human ARF-1 recombinant protein, (Cat#:ab123538 )
Human ARF-3 recombinant protein, (Cat#:ab113581 ) -
ICC/IF image of ab108347 stained CaCo2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108347 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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All lanes : Anti-ARF1 + ARF3 antibody [EPR443] (ab108347) at 1/1000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human fetal brain tissue lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 21 kDa -
Immunohistochemical analysis of ARF1 in paraffin embedded Human breast ductal carcinoma tissue, using ab108347 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of ARF1 in paraffin embedded Human colonic adenocarcinoma tissue, using ab108347 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab108347 は 7 報の論文で使用されています。
- Liu Q et al. Histone demethylase PHF8 drives neuroendocrine prostate cancer progression by epigenetically upregulating FOXA2. J Pathol 253:106-118 (2021). PubMed: 33009820
- Liu J et al. ARF3 inhibits proliferation and promotes apoptosis in gastric cancer by regulating AKT and ERK pathway. Acta Biochim Pol 68:223-229 (2021). PubMed: 33847108
- Zhu T et al. TRPM2 channel regulates cytokines production in astrocytes and aggravates brain disorder during lipopolysaccharide-induced endotoxin sepsis. Int Immunopharmacol 75:105836 (2019). PubMed: 31450153
- Rafiq NB et al. Podosome assembly is controlled by the GTPase ARF1 and its nucleotide exchange factor ARNO. J Cell Biol 216:181-197 (2017). WB . PubMed: 28007915
- Eiseler T et al. Protein Kinase D2 Assembles a Multiprotein Complex at the Trans-Golgi Network to Regulate Matrix Metalloproteinase Secretion. J Biol Chem 291:462-77 (2016). PubMed: 26507660
- Schultz ML et al. CLN3 deficient cells display defects in the ARF1-Cdc42 pathway and actin-dependent events. PLoS One 9:e96647 (2014). WB ; Mouse . PubMed: 24792215
- Jung JJ et al. Secretion of soluble vascular endothelial growth factor receptor 1 (sVEGFR1/sFlt1) requires Arf1, Arf6, and Rab11 GTPases. PLoS One 7:e44572 (2012). PubMed: 22962618