Anti-ZEB1 抗体 (ab124512)
Key features and details
- Rabbit polyclonal to ZEB1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-ZEB1 antibody
ZEB1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ZEB1 -
由来種
Rabbit -
特異性
ab124512 is predicted not to crossreact with ZEB2. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human ZEB1 (internal sequence).
Database link: P37275 -
ポジティブ・コントロール
- WB: HeLa whole cell lysate (ab150035). ICC: HeLa and K562 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124512の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 124 kDa.
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特記事項 |
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ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 124 kDa. |
ターゲット情報
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機能
Inhibits interleukin-2 (IL-2) gene expression. May be responsible for transcriptional repression of the IL-2 gene. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. -
組織特異性
Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. -
関連疾患
Defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3 (PPCD3) [MIM:609141]. PPCD is a rare disease involving metaplasia and overgrowth of corneal endothelial cells. In patients with PPCD, these cells manifest in an epithelial morphology and gene expression pattern, produce an aberrant basement membrane, and, sometimes, spread over the iris and nearby structures in a way that increases the risk for glaucoma.
Defects in ZEB1 are the cause of corneal dystrophy Fuchs endothelial type 6 (FECD6) [MIM:613270]. It is an ocular disorder caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition. -
配列類似性
Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
Contains 7 C2H2-type zinc fingers.
Contains 1 homeobox DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6935 Human
- Omim: 189909 Human
- SwissProt: P37275 Human
- Unigene: 124503 Human
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別名
- AREB 6 antibody
- AREB6 antibody
- BZP antibody
see all
画像
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Anti-ZEB1 antibody (ab124512) at 1 µg/ml + HeLa cell lysate at 15 µg
Predicted band size: 124 kDa -
Immunocytochemistry of HeLa cells labeling ZEB1 with Anti-ZEB1 antibody (ab124512) at 20μg/ml.
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Immunocytochemistry/Immunofluorescence of ZEB1 in HeLa cells using ab124512 at 20µg/ml.
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Immunocytochemical analysis of K562 cells labelling ZEB1 with ab124512 at 2.5μg/mL.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (38)
ab124512 は 38 報の論文で使用されています。
- Nam HJ et al. GPR110 promotes progression and metastasis of triple-negative breast cancer. Cell Death Discov 8:271 (2022). PubMed: 35614051
- Zhao X et al. Cytoplasmic Localization Isoform of Cyclin Y Enhanced the Metastatic Ability of Lung Cancer via Regulating Tropomyosin 4. Front Cell Dev Biol 9:684819 (2021). PubMed: 34222253
- Yao S et al. Monensin suppresses cell proliferation and invasion in ovarian cancer by enhancing MEK1 SUMOylation. Exp Ther Med 22:1390 (2021). PubMed: 34650638
- Kim YJ et al. Suppression of breast cancer progression by FBXL16 via oxygen-independent regulation of HIF1α stability. Cell Rep 37:109996 (2021). PubMed: 34818544
- Zhao L et al. lncRNA SNHG17 promotes pancreatic carcinoma progression via cross-talking with miR-942. Am J Transl Res 13:1037-1050 (2021). PubMed: 33841638