The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 45 kDa).
Abcam recommends using 3% milk as the blocking agent.
Required for consolidation of synaptic plasticity as well as formation of long-term memory. Regulates endocytosis of AMPA receptors in response to synaptic activity. Required for homeostatic synaptic scaling of AMPA receptors (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration.
Belongs to the ARC/ARG3.1 family.
Cytoplasm > cytoskeleton. Endosome. Cytoplasmic vesicle > secretory vesicle > acrosome. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Cell projection > dendritic spine. Cell junction > synapse. Associated with the cell cortex of neuronal soma and dendrites. Enriched in postsynaptic density of dendritic spines. Associated with the sperm tail (By similarity). Enriched on the plasma membrane.
All lanes : Anti-Arc antibody (ab23382) at 1 µg/ml
Lane 1 : Hippocampus (Mouse) Tissue Lysate blocked with 5% BSA Lane 2 : Rat Hippocampus Tissue Lysate blocked with 5% BSA Lane 3 : Hippocampus (Mouse) Tissue Lysate blocked with 5% milk Lane 4 : Rat Hippocampus Tissue Lysate blocked with 5% milk Lane 5 : Hippocampus (Mouse) Tissue Lysate blocked with 3% milk Lane 6 : Rat Hippocampus Tissue Lysate blocked with 3% milk Lane 7 : Hippocampus (Mouse) Tissue Lysate blocked with 1% milk Lane 8 : Rat Hippocampus Tissue Lysate blocked with 1% milk
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Wu H et al. Salicylate-induced changes in immediate-early genes in the hippocampal CA1 area. Mol Med Rep12:1625-30 (2015).
Read more (PubMed: 25873216) »
Hu SS et al. Expression of immediate-early genes in the inferior colliculus and auditory cortex in salicylate-induced tinnitus in rat. Eur J Histochem58:2294 (2014).
Read more (PubMed: 24704997) »