製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ARAP1
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Cow, Dog, Pig, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 1160-1210 of Human ARAP1 (NP_056057.1).

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab99382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 136 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能
    Phosphatidylinositol-3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol-3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5.
  • 組織特異性
    Detected in heart, skeletal muscle, spleen, kidney, liver, placenta, lung, peripheral blood leukocytes, adrenal gland, bone marrow, brain, lymph node, mammary gland, prostate, spinal cord, stomach, thyroid and trachea.
  • 配列類似性
    Contains 1 Arf-GAP domain.
    Contains 4 PH domains.
    Contains 1 Ras-associating domain.
    Contains 1 Rho-GAP domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • 細胞内局在
    Cytoplasm. Golgi apparatus > Golgi stack membrane. Cell membrane. Associated with Golgi stacks in resting cells. Throughout the cytoplasm and in surface protrusion in cells that are in the process of attaching to a surface and spreading.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2410002L19Rik antibody
    • ANK repeat and PH domain-containing protein 1 antibody
    • ARAP 1 antibody
    • Arap1 antibody
    • ARAP1_HUMAN antibody
    • Arf GAP Rho GAP ankyrin repeat and pleckstrin homology domains containing protein 1 antibody
    • Arf GAP Rho GAP ankyrin repeat and pleckstrin homology domain containing protein 1 antibody
    • Arf-GAP with Rho-GAP domain antibody
    • CENT D2 antibody
    • Centaurin delta 2 antibody
    • Centaurin delta2 antibody
    • Centaurin-delta-2 antibody
    • CENTD 2 antibody
    • CENTD2 antibody
    • Cnt d2 antibody
    • Cnt-d2 antibody
    • Cntd2 antibody
    • KIAA0782 antibody
    • MGC176140 antibody
    • mKIAA0782 antibody
    see all

画像

  • All lanes : Anti-ARAP1 antibody (ab99382) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 136 kDa


    Exposure time : 30 seconds
  • Detection of ARAP1 by Western Blot of Immunprecipitate.
    ab99382 at 1µg/ml staining ARAP1 in HeLa whole cell lysate immunoprecipitated using ab99382 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

参考文献

ab99382 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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