Anti-Aquaporin 8 抗体 (ab15124)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Aquaporin 8
  • 特異性
    The 16 AA peptide was found unique to AQP8 without significant homology to any other AQPs
  • アプリケーション
    適用あり: WB, ELISAmore details
    適用なし: IHC
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    A 16 aa synthetic peptide (Rat) (C terminal). The domain is predicted to be cytoplasmic.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    ELISA: 1/10000 - 1/50000.
    WB: 1/1000 - 1/5000. Predicted molecular weight: 29 kDa. Use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations is recommended in order to suppress non-specific bands.

    Is unsuitable for IHC.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Forms a water-specific channel; mercury-sensitive. Not permeable to glycerol or urea.
    • 組織特異性
      Expressed only in pancreas and colon.
    • 配列類似性
      Belongs to the MIP/aquaporin (TC 1.A.8) family.
    • ドメイン
      Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
    • 細胞内局在
      Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AQP 8 antibody
      • AQP-8 antibody
      • AQP8 antibody
      • AQP8_HUMAN antibody
      • Aquaporin 8 antibody
      • Aquaporin-8 antibody
      • Aquaporin8 antibody
      see all

    プロトコール

    参考文献

    ab15124 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (colon)
    Loading amount
    60 µg
    Specification
    colon
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 21 2011

    Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab15124. Are you using human samples? What dilutions of ab15124 have you tried? At this point I would suggest using it at a 1:1000 dilution and incubate overnigh...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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