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RabMAb

Anti-Aquaporin 5 抗体 [EPR3747] (ab92320)

製品の概要

  • 製品名
    Anti-Aquaporin 5 antibody [EPR3747]
    Aquaporin 5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3747] to Aquaporin 5
  • アプリケーション
    適用あり: Flow Cyt, WB, IHC-P, ICC/IFmore details
    適用なし: IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues within Human Aquaporin 5

  • ポジティブ・コントロール
    • Fetal lung and SW480 cell lysates, Human lung tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.Can be blocked with Aquaporin 5 peptide (ab214008).
IHC-P 1/100 - 1/250. Antigen retrieval is recommended before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended as stronger signals have been found using these.
ICC/IF 1/100 - 1/250.
  • 追加情報
    Is unsuitable for IP.
  • ターゲット情報

    • 機能
      Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. Required for TRPV4 activation by hypotonicity (PubMed:16571723). Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells (PubMed:16571723).
    • 関連疾患
      Keratoderma, palmoplantar, Bothnian type
    • 配列類似性
      Belongs to the MIP/aquaporin (TC 1.A.8) family.
    • ドメイン
      Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
    • 細胞内局在
      Apical cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AQP 5 antibody
      • AQP-5 antibody
      • AQP5 antibody
      • AQP5_HUMAN antibody
      • Aquaporin 5 antibody
      • Aquaporin-5 antibody
      see all

    画像

    • Flow cytometry analysis of SW480 human colorectal adenocarcinoma ) cells labeling with unpurified ab92320 at 1/200 dilution ( 1ug/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077) )(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as a isotype control.Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

    • ab92320 showing positive staining in Normal colon tissue.

    • All lanes : Anti-Aquaporin 5 antibody [EPR3747] (ab92320) at 1/1000 dilution

      Lane 1 : Fetal lung lysate
      Lane 2 : SW480 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit IgG at 1/1000 dilution

      Predicted band size : 28 kDa
    • ab92320 at 1/100 dilution, staining Aquaporin 5 in paraffin embedded Human lung tissue
    • ab92320 showing positive staining in Normal stomach tissue.

    • ab92320 showing negative staining in Normal brain tissue.

    参考文献

    This product has been referenced in:
    • Wolf L  et al. Receptor for advanced glycation endproducts (RAGE) maintains pulmonary structure and regulates the response to cigarette smoke. PLoS One 12:e0180092 (2017). WB ; Mouse . Read more (PubMed: 28678851) »
    • Yu CJ  et al. Effects of glucocorticoid on the expression and regulation of aquaporin 5 in the paranasal sinus of rats with chronic rhinosinusitis. Exp Ther Med 13:1753-1756 (2017). Read more (PubMed: 28565763) »

    See all 10 Publications for this product

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (intervertebral disc)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    intervertebral disc
    Blocking step
    1% BSA in 75% TBS 25% serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 25% · Temperature: 18°C
    Fixative
    10% neutral buffered formalin
    Username

    Abcam user community

    Verified customer

    投稿 Jun 05 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Lung)
    Gel Running Conditions
    Reduced Denaturing (12% Bis-Tris NuPAGE)
    Loading amount
    50 µg
    Specification
    Lung
    Blocking step
    Odyssey TBS Blocking Buffer (proprietary) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
    Username

    Pete Serbedzija

    Verified customer

    投稿 Apr 08 2016

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (AQP5-EGFP transfected cells / rat lung slices)
    Permeabilization
    Yes - 0,2 % Saponin
    Specification
    AQP5-EGFP transfected cells / rat lung slices
    Blocking step
    Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Oct 01 2015

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Zytomed Systems HRP Polymer Kit Blocking Solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
    Antigen retrieval step
    None
    Sample
    Human Tissue sections (Lung tissue)
    Specification
    Lung tissue
    Permeabilization
    No
    Fixative
    HOPE-Fixation
    Username

    Abcam user community

    Verified customer

    投稿 Mar 12 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (A549)
    Specification
    A549
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.25% Triton X-100/PBS
    Username

    Abcam user community

    Verified customer

    投稿 Apr 05 2013

    Thank you for reply.

    I am afraid I will not be able to offer a discount code for the Anti-Prosurfactant Protein B antibody (ab104813) as I am not sure if the antibody is likely to cross react with the chicken protein.

    I can provide ...

    Read More

    Thank you for contacting us and sorry for the delay in getting back to you.

    In regards to the Anti-Prosurfactant Protein B antibody (ab104813), I was only able to locate a chicken protein, with the SwissProt reference http://www.uniprot.org/u...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    Human Cell (primary alveolar epithelial cells)
    Specification
    primary alveolar epithelial cells
    Fixative
    HOPE
    Permeabilization
    No
    Blocking step
    Zytomed Systems HRP Kit Blocking solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Jul 31 2012

    Thank you for your enquiry.

    I can confirm that ab92320 antibody is sold as whole antiserum / ascites / tissue culture supernatant. Unpurified antibodies, such as those sold as tissue culture supernatant will not have a concentration stated on ...

    Read More

    Thank you for your inquiry.

    I can confirm that this antibody does not react with mouse and rat brain, heart, kidney and spleen samples as tested by our lab in WB.

    I hope this information is helpful and wish you good luck with your e...

    Read More

    1-10 of 13 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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